An Integrated Transcriptome Atlas of Embryonic Hair Follicle Progenitors, Their Niche, and the Developing Skin
| Autor: | Michael Rendl, Nicholas Heitman, Amélie Rezza, Carlos Clavel, Rachel Sennett, Zichen Wang, Cristina Sicchio, Avi Ma'ayan, Laura Grisanti, Nataly Roitershtein, Ka-Wai Mok |
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| Rok vydání: | 2015 |
| Předmět: |
Keratinocytes
Cellular differentiation Organogenesis Population Biology Bioinformatics General Biochemistry Genetics and Molecular Biology Article Transcriptome 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Stem Cell Niche education Molecular Biology Cells Cultured 030304 developmental biology Skin 0303 health sciences education.field_of_study integumentary system Stem Cells Cell Differentiation Cell Biology Hair follicle Embryonic stem cell Cell biology Dermal papillae medicine.anatomical_structure Axon guidance Stem cell Hair Follicle 030217 neurology & neurosurgery Developmental Biology Signal Transduction |
| Zdroj: | Developmental cell. 34(5) |
| ISSN: | 1878-1551 |
| Popis: | SummaryDefining the unique molecular features of progenitors and their niche requires a genome-wide, whole-tissue approach with cellular resolution. Here, we co-isolate embryonic hair follicle (HF) placode and dermal condensate cells, precursors of adult HF stem cells and the dermal papilla/sheath niche, along with lineage-related keratinocytes and fibroblasts, Schwann cells, melanocytes, and a population inclusive of all remaining skin cells. With next-generation RNA sequencing, we define gene expression patterns in the context of the entire embryonic skin, and through transcriptome cross-comparisons, we uncover hundreds of enriched genes in cell-type-specific signatures. Axon guidance signaling and many other pathway genes are enriched in multiple signatures, implicating these factors in driving the large-scale cellular rearrangements necessary for HF formation. Finally, we share all data in an interactive, searchable companion website. Our study provides an overarching view of signaling within the entire embryonic skin and captures a molecular snapshot of HF progenitors and their niche. |
| Databáze: | OpenAIRE |
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