Very long chain fatty acid metabolism is required in acute myeloid leukemia
| Autor: | Jerry Vockley, Kristin J Hope, Kevin A Rea, Tariq A. Akhtar, Aaron D. Schimmer, Al-Walid Mohsen, Nawaz Ahmed, Paul A. Spagnuolo, Angelo D'Alessandro, Richard W. Smith, Matthew Tcheng, David A. Hess, Eric Bohrnsen, Mark D. Minden, Preethi Jayanth, Alessia Roma |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Citric Acid Cycle Immunology Very long chain fatty acid Mitochondrion Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor hemic and lymphatic diseases medicine Humans Glycolysis Beta oxidation Myeloid Neoplasia Chemistry Acyl-CoA Dehydrogenase Long-Chain Fatty Acids Myeloid leukemia Ketone Oxidoreductases Cell Biology Hematology medicine.disease Neoplasm Proteins Citric acid cycle Leukemia Myeloid Acute Leukemia 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Adenosine triphosphate |
| Zdroj: | Blood Paediatrics Publications |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.2020008551 |
| Popis: | Acute myeloid leukemia (AML) cells have an atypical metabolic phenotype characterized by increased mitochondrial mass, as well as a greater reliance on oxidative phosphorylation and fatty acid oxidation (FAO) for survival. To exploit this altered metabolism, we assessed publicly available databases to identify FAO enzyme overexpression. Very long chain acyl-CoA dehydrogenase (VLCAD; ACADVL) was found to be overexpressed and critical to leukemia cell mitochondrial metabolism. Genetic attenuation or pharmacological inhibition of VLCAD hindered mitochondrial respiration and FAO contribution to the tricarboxylic acid cycle, resulting in decreased viability, proliferation, clonogenic growth, and AML cell engraftment. Suppression of FAO at VLCAD triggered an increase in pyruvate dehydrogenase activity that was insufficient to increase glycolysis but resulted in adenosine triphosphate depletion and AML cell death, with no effect on normal hematopoietic cells. Together, these results demonstrate the importance of VLCAD in AML cell biology and highlight a novel metabolic vulnerability for this devastating disease. |
| Databáze: | OpenAIRE |
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