The antagonic behavior of GPIHBP1 between EAT and circulation does not reflect lipolytic enzymes levels in the tissue and serum from coronary patients
| Autor: | Graciela López, Katsuyuki Nakajima, Masami Murakami, Laura Schreier, Takumi Nagasawa, Julio Baldi, Gabriela Berg, Verónica Miksztowicz, Magalí Barchuk, Miguel Rubio |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Endothelial lipase medicine.medical_specialty Clinical Biochemistry Coronary Artery Disease Biochemistry Coronary artery disease 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Humans cardiovascular diseases Receptors Lipoprotein Lipoprotein lipase Triglyceride business.industry digestive oral and skin physiology Biochemistry (medical) GPIHBP1 General Medicine medicine.disease Coronary arteries Lipoprotein Lipase 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Adipose Tissue 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) business Artery Lipoprotein |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 510 |
| ISSN: | 1873-3492 |
| Popis: | Coronary artery disease (CAD) is the leading cause of morbidity and mortality worldwide. Recently, triglyceride rich lipoproteins are proposed to contribute to CAD risk; its concentrations would be partly determined by lipoprotein lipase (LPL) and endothelial lipase (EL). Epicardial adipose tissue (EAT), a visceral AT surrounding myocardium and coronary arteries, emerged as an important actor in CAD; the increase in its volume could be a consequence of LPL and EL. Circulating enzymes levels would be conditioned by local tissue factors. Our aim was to evaluate LPL, EL and their regulators levels in serum and EAT from CAD patients, searching for possible parallelisms and their role in the lipoprotein profile.In serum, EAT and subcutaneous AT (SAT) from patients undergoing coronary artery bypass graft (CABG, n = 25) or valve replacement (No CABG, n = 25), LPL, EL and glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein-1 (GPIHBP1) expression were evaluated. Besides, Apoprotein (Apo)CII, CIII and AV were determined in serum, along with lipoprotein profile.Insulin-resistance markers were higher in CABG (p 0.05). Serum LPL levels were decreased (p = 0.045), while EL levels increased (p 0.001) in CABG, without differences in EAT or SAT. Circulating GPIHBP1 concentrations were decreased in CABG (p = 0.047), while EAT GPIHBP1 expression was increased (p 0.001). ApoCII and ApoAV concentrations were higher in CABG (p = 0.016 and p = 0.047, respectively), without differences in ApoCIII concentrations between groups.In EAT, LPL and EL protein levels were not changed in CAD, although GPIHBP1 protein levels were higher. EAT would be a minor contributor to the circulating levels of the enzymes. |
| Databáze: | OpenAIRE |
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