The inositol phosphatase MTMR4 is a novel target of the ubiquitin ligase Nedd4

Autor:	Jane Batt, Judy Correa, Pamela J. Plant, Neil M. Goldenberg, James R. Bain
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	muscle atrophy Male Myotubularin Nedd4 Ubiquitin Protein Ligases NEDD4 Biochemistry myotubularin-related protein 4 (MTMR4) 0302 clinical medicine Ubiquitin GST glutathione transferase inositol phosphatase Interactor XLMTM X-linked myotubular myopathy GFP green fluorescent protein 0303 health sciences HA haemagglutinin DMEM Dulbecco's modified Eagle's medium PX phox homology domain DSP dual-specificity phosphatase ENaC epithelial sodium channel Muscles Protein Tyrosine Phosphatases Non-Receptor Muscle atrophy Ubiquitin ligase Cell biology myotubularin MTMR4 myotubularin-related protein 4 CCD charge-coupled device medicine.symptom PTP protein tyrosine phosphatase Protein Binding HPRT hypoxanthine–guanine phosphoribosyltransferase Inositol phosphatase Endosome Nedd4 neural-precursor-cell-expressed developmentally down-regulated 4 Ubiquitin-Protein Ligases Blotting Western LAMP lysosome-associated membrane protein Accelerated Publication macromolecular substances Biology neural-precursor-cell-expressed developmentally down-regulated 4 (Nedd4) ubiquitin ligase Cell Line 03 medical and health sciences HEK human embryonic kidney h human medicine Animals Humans Immunoprecipitation Molecular Biology 030304 developmental biology Endosomal Sorting Complexes Required for Transport Ubiquitination Cell Biology BCA bicinchoninic acid Protein Structure Tertiary Rats HECT homologous with E6-associated protein C-terminus biology.protein 030217 neurology & neurosurgery HeLa Cells
Zdroj:	Biochemical Journal
ISSN:	1470-8728 0264-6021
Popis:	The inositol phosphatase, MTMR4 (myotubularin-related protein 4), was identified as a novel interactor of the ubiquitin ligase Nedd4 (neural-precursor-cell-expressed developmentally down-regulated 4). hMTMR4 (human MTMR4) and Nedd4 co-immunoprecipitated and co-localized to late endosomes. The PY (Pro-Tyr) motif of hMTMR4 binds to WW (Trp-Trp) domains of hNedd4. MTMR4 expression was decreased in atrophying muscle, whereas Nedd4 expression was increased and hMTMR4 was ubiquitinated by hNedd4, suggesting that this novel interaction may underlie the biological process of muscle breakdown.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4439799fa09014a70381e2a7c925e750 http://europepmc.org/articles/PMC2657291 Zobrazit plný text záznamu