Extratumoral Heme Oxygenase-1 (HO-1) Expressing Macrophages Likely Promote Primary and Metastatic Prostate Tumor Growth
| Autor: | Elin Thysell, Maria Nilsson, Sofia Halin Bergström, Anders Bergh, Anders Widmark, Emma Jernberg, Pernilla Wikström, Pär Stattin, Hanibal Hani Adamo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology Gene Expression lcsh:Medicine Epithelium White Blood Cells Prostate cancer 0302 clinical medicine Animal Cells Prostate Medicine and Health Sciences Macrophage Neoplasm Metastasis lcsh:Science Staining Multidisciplinary Invasive Tumors Prostate Cancer Prostate Diseases Cell Staining Neoplasm Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Immunohistochemistry Anatomy Cellular Types Research Article PCA3 medicine.medical_specialty Urology Immune Cells Immunology Research and Analysis Methods Gene Expression Regulation Enzymologic 03 medical and health sciences Exocrine Glands Genetics medicine Animals Humans Cancer och onkologi Blood Cells business.industry Macrophages lcsh:R Cancers and Neoplasms Biology and Life Sciences Prostatic Neoplasms Cancer Epithelial Cells Cell Biology medicine.disease Rats Heme oxygenase Androgen receptor Genitourinary Tract Tumors Biological Tissue 030104 developmental biology Metastatic Tumors Specimen Preparation and Treatment Cancer and Oncology Heme Oxygenase (Decyclizing) Prostate Gland lcsh:Q business Heme Oxygenase-1 |
| Zdroj: | PLoS ONE, Vol 11, Iss 6, p e0157280 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Aggressive tumors induce tumor-supporting changes in the benign parts of the prostate. One factor that has increased expression outside prostate tumors is hemoxygenase-1 (HO-1). To investigate HO-1 expression in more detail, we analyzed samples of tumor tissue and peritumoral normal prostate tissue from rats carrying cancers with different metastatic capacity, and human prostate cancer tissue samples from primary tumors and bone metastases. In rat prostate tumor samples, immunohistochemistry and quantitative RTPCR showed that the main site of HO-1 synthesis was HO-1(+) macrophages that accumulated in the tumor-bearing organ, and at the tumor-invasive front. Small metastatic tumors were considerably more effective in attracting HO-1(+) macrophages than larger non-metastatic ones. In clinical samples, accumulation of HO-1(+) macrophages was seen at the tumor invasive front, almost exclusively in high-grade tumors, and it correlated with the presence of bone metastases. HO-1(+) macrophages, located at the tumor invasive front, were more abundant in bone metastases than in primary tumors. HO-1 expression in bone metastases was variable, and positively correlated with the expression of macrophage markers but negatively correlated with androgen receptor expression, suggesting that elevated HO-1 could be a marker for a subgroup of bone metastases. Together with another recent observation showing that selective knockout of HO-1 in macrophages reduced prostate tumor growth and metastatic capacity in animals, the results of this study suggest that extratumoral HO-1(+) macrophages may have an important role in prostate cancer. |
| Databáze: | OpenAIRE |
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