Dual Function of NRP1 in Axon Guidance and Subcellular Target Recognition in Cerebellum
| Autor: | Jean-Michel Cioni, Fabrice Ango, Andrea B. Huber, Céline Jahannault-Talignani, Véronique Saywell, Catherine Sarrailh-Faivre, Alexandre Dayer, Rosa Eva Huettl, Christelle Cadilhac, Ludovic Telley |
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| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cerebellum Neurogenesis/physiology [SDV]Life Sciences [q-bio] Synaptogenesis Nerve Growth Factors/metabolism Purkinje Cells/physiology semaphorin axon initial segment Synapse ddc:616.89 Purkinje Cells GABA Axon GABAergic Neurons ComputingMilieux_MISCELLANEOUS General Neuroscience GABAergic Neurons/physiology Axon Guidance adhesion medicine.anatomical_structure Editorial Synapses/physiology Cell Adhesion Molecules/metabolism Neurogenesis Semaphorin-3A/physiology CHO Cells Biology neurofascin186 03 medical and health sciences Cricetulus Semaphorin medicine Animals Humans Nerve Growth Factors Axon Guidance/physiology Semaphorin-3A Cerebellum/cytology/growth & development Axon initial segment Coculture Techniques Neuropilin-1 ddc:616.8 Neuropilin-1/physiology 030104 developmental biology nervous system Synapses Axon guidance Soma Neuroscience Cell Adhesion Molecules |
| Zdroj: | Oncotarget Neuron 91, 1276-1291 (2016) Neuron Neuron, Elsevier, 2016, 91 (6), pp.1276--91. ⟨10.1016/j.neuron.2016.08.015⟩ Neuron, Vol. 91, No 6 (2016) pp. 1276-1291 Neuron, 2016, 91 (6), pp.1276--91. ⟨10.1016/j.neuron.2016.08.015⟩ |
| ISSN: | 1097-4199 0896-6273 |
| Popis: | Subcellular target recognition in the CNS is the culmination of a multiple-step program including axon guidance, target recognition, and synaptogenesis. In cerebellum, basket cells (BCs) innervate the soma and axon initial segment (AIS) of Purkinje cells (PCs) to form the pinceau synapse, but the underlying mechanisms remain incompletely understood. Here, we demonstrate that neuropilin-1 (NRP1), a Semaphorin receptor expressed in BCs, controls both axonal guidance and subcellular target recognition. We show that loss of Semaphorin 3A function or specific deletion of NRP1 in BCs alters the stereotyped organization of BC axon and impairs pinceau synapse formation. Further, we identified NRP1 as a trans-synaptic binding partner of the cell adhesion molecule neurofascin-186 (NF186) expressed in the PC AIS during pinceau synapse formation. These findings identify a dual function of NRP1 in both axon guidance and subcellular target recognition in the construction of GABAergic circuitry. |
| Databáze: | OpenAIRE |
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