Preparation of Protein Conjugates via Homobifunctional Diselenoester Cross-Linker
| Autor: | Xiang-Zhao Chen, Jian Wang, Ling-Ming Xin, Ze Lei, Xu-Guang Yin, Jing-Jing Du, Jun Guo, Xiao-Fei Gao, Xiao-Kang Zhang, Zheng Liu |
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| Rok vydání: | 2016 |
| Předmět: |
Ovalbumin
Stereochemistry Adipates Peptide 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Organoselenium Compounds Reactivity (chemistry) Physical and Theoretical Chemistry chemistry.chemical_classification Vaccines Synthetic Adipic acid 010405 organic chemistry Organic Chemistry Serum Albumin Bovine History 20th Century Cross-Linking Reagents 0104 chemical sciences chemistry Covalent bond Haptens Linker Hapten Conjugate |
| Zdroj: | Organic Letters. 18:5796-5799 |
| ISSN: | 1523-7052 1523-7060 |
| DOI: | 10.1021/acs.orglett.6b02568 |
| Popis: | Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts of N-hydroxysuccinimide (NHS) and p-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency. |
| Databáze: | OpenAIRE |
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