Methadone concentrations in blood, plasma, and oral fluid determined by isotope-dilution gas chromatography–mass spectrometry

Autor: Shiao-Ping Huang, Yu-Shan Wang, Dong-Liang Lin, A. Wayne Jones, Ya-Ching Hsu, Mei-Han Huang, Hsu-Chun Liu, Shu-Ching Yang, Tai-Jui Chen, Bud-Gen Chen, Ray H. Liu
Rok vydání: 2012
Předmět:
Zdroj: Analytical and Bioanalytical Chemistry. 405:3921-3928
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-012-6460-2
Popis: Methadone (MTD) is widely used for detoxification of heroin addicts and also in pain management programs. Information about the distribution of methadone between blood, plasma, and alternative specimens, such as oral fluid (OF), is needed in clinical, forensic, and traffic medicine when analytical results are interpreted. We determined MTD and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in blood, plasma, blood cells, and OF by gas chromatography–mass spectrometry (GC-MS) after adding deuterium-labeled internal standards. The analytical limits of quantitation for MTD and EDDP by this method were 20 and 3 ng/mL, respectively. The amounts of MTD and EDDP were higher in plasma (80.4 % and 76.5 %) compared with blood cells (19.6 % and 23.5 %) and we found that repeated washing of blood cells with phosphate–buffered saline increased the amounts in plasma (93.6 % and 88.6 %). Mean plasma/blood concentration ratios of MTD and EDDP in spiked samples (N = 5) were 1.27 and 1.21, respectively. In clinical samples from patients (N = 46), the concentrations of MTD in plasma and whole blood were highly correlated (r = 0.92, p
Databáze: OpenAIRE
Externí odkaz: