Three-dimensional co-culture model of chronic lymphocytic leukemia bone marrow microenvironment predicts patient-specific response to mobilizing agents
| Autor: | Desirée Zambroni, Paolo Ghia, Lydia Scarfò, Maurilio Ponzoni, Elisabetta Ferrero, Francesca Vittoria Sbrana, Lucia Bongiovanni, Cristina Scielzo, Kostas Stamatopoulos, Federica Barbaglio, Valeria R. Caiolfa, Luca Pavesi, Daniela Belloni |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Chronic lymphocytic leukemia Cell Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo immune system diseases Bone Marrow hemic and lymphatic diseases medicine Tumor Microenvironment Humans Cytoskeleton Hematology medicine.disease Leukemia Lymphocytic Chronic B-Cell Pathophysiology Coculture Techniques medicine.anatomical_structure Pyrimidines chemistry Ibrutinib Cancer research Pyrazoles Bone marrow 030215 immunology |
| Zdroj: | Haematologica |
| ISSN: | 1592-8721 |
| Popis: | Chronic lymphocytic leukemia (CLL) cells disseminate into supportive tissue microenvironments. To investigate the mechanisms involved in leukemic cell tissue retention we developed a threedimensional bone marrow (BM) microenvironment that recreates the interactions between CLL and BM stromal cells inside a scaffold within a bioreactor. Our system allows the parallel analysis of CLL cells retained inside the scaffold and those released in the presence/absence of pharmacological agents, mimicking tissue and circulating cell compartments, respectively. CLL cells can be retained within the scaffold only in the presence of microenvironmental elements, which through direct contact downregulate the expression of HS1 cytoskeletal protein in CLL cells. Consistent with this, the expression of HS1 was lower in CLL cells obtained from patients’ BM than in CLL cells circulating in the peripheral blood. Moreover, we demonstrate that CLL cells with inactive HS1, impaired cytoskeletal activity and a more aggressive phenotype are more likely to be retained within the scaffold despite the presence of ibrutinib, whose mobilizing effect is mainly exerted on those with active HS1, ensuing dynamic cytoskeletal activity. This differential effect would not otherwise be assessable in a traditional two-dimensional system and may underlie a distinctive resistance of single CLL clones. Notably, CLL cells mobilized in the peripheral blood of patients during ibrutinib therapy exhibited activated HS1, underscoring that our model reliably mirrors the in vivo situation. The three-dimensional model described herein is suitable for reproducing and identifying critical CLL-BM interactions, opening the way to pathophysiological studies and the evaluation of novel targeted therapies in an individualized manner. |
| Databáze: | OpenAIRE |
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