Molecular cloning and expression of a novel human aquaporin from adipose tissue with glycerol permeability
| Autor: | Kousaku Okubo, Kenichi Matsubara, Yuji Matsuzawa, Narihiro Ishida, Hiroshi Kuriyama, Shiro Mita, Ikko Ohno, Shoko Kawamoto |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Glycerol FGF21 Cell Membrane Permeability DNA Complementary Xenopus Molecular Sequence Data Biophysics Glycerol transport Aquaporin Adipose tissue Biology Aquaporins Biochemistry Polymerase Chain Reaction Ion Channels Gene product Consensus Sequence Animals Humans Northern blot Amino Acid Sequence RNA Messenger Cloning Molecular Protein kinase A Molecular Biology Gene Library Aquaporin 3 Base Sequence Sequence Homology Amino Acid Cell Biology Cyclic AMP-Dependent Protein Kinases Transmembrane domain Adipose Tissue Oocytes Female Sequence Alignment |
| Zdroj: | Biochemical and biophysical research communications. 241(1) |
| ISSN: | 0006-291X |
| Popis: | In a systematic analysis of genes expressed in human adipose tissue, we detected a novel gene that is expressed uniquely in adipose tissue. The sequence showed that it encodes a 342-amino-acid protein containing six putative transmembrane domains, and is a new member of the aquaporin family of water-selective membrane channels. We named this gene aquaporin 9. It features a cyclic-AMP protein kinase phosphorylation consensus site in the NH3-terminal domain. Expression of the cRNA inXenopusoocytes yielded a 7-fold increase in osmotic water permeability blocked by 0.3 mM HgCl2, and also facilitated the uptake of glycerol. Northern blot analysis demonstrated that the mRNA is abundant in adipose tissue, but not in other tissues. Thus, this gene product may participate in glycerol transport in adipocytes. |
| Databáze: | OpenAIRE |
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