Effect of reserpine on the histochemical and biochemical properties of rat intestinal mucin
| Autor: | Nusrath W. Roomi, William Kuhns, Ramin Khorasani, Gordon G. Forstner, Janet F. Forstner |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Sulfotransferase Reserpine Clinical Biochemistry Sulfur Radioisotopes Pathology and Forensic Medicine Sulfation Reference Values Internal medicine Intestine Small medicine Animals Intestinal Mucosa Molecular Biology chemistry.chemical_classification Chemistry Sulfates Mucin Body Weight Mucins Rats Inbred Strains Oligosaccharide Small intestine Rats Kinetics medicine.anatomical_structure Endocrinology Cardiovascular agent Sulfotransferases Glycoprotein medicine.drug |
| Zdroj: | Experimental and molecular pathology. 54(2) |
| ISSN: | 0014-4800 |
| Popis: | Biochemical and histochemical parameters of intestinal mucins were examined in control and reserpine-treated rats. An assay for intestinal mucin sulfotransferase was developed and the activity shown to increase 3,4 times over control levels in rats given intraperitonal reserpine (0.5 mg/kg body wt) daily for 7 days. Histochemical staining of intestinal sections revealed an increase in sulfomucins in goblet cells of reserpine-treated rats. The effects were prominent as early as 1 day following injection, particularly in the distal third of the small intestine, and during the next 6 days these changes spread progressively to the middle and proximal thirds. After 3 days of treatment mucins were purified from each intestinal segment and compared to control mucins with respect to composition and [ 35 S]NaSO 4 incorporation. Although individual amino acid and carbohydrate molar ratios were unchanged, the total carbohydrate and sulfate content of mucins in treated animals was elevated (two to three times above control) in the middle and distal thirds of the intestine. In vivo [ 35 S]SO 4 incorporation into these mucins was also proportionately elevated, and was targetted to O -linked oligosaccharide side chains. These findings are consistent with an action of reserpine causing an increased production of mucin which is enriched in glycoprotein components bearing sulfated oligosaccharide chains. The relevance of these findings to the production of hypersulfated and hyperglycosylated mucins in cystic fibrosis is discussed. |
| Databáze: | OpenAIRE |
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