Glycosidic bond specificity of glucansucrases: on the role of acceptor substrate binding residues
| Autor: | Hans Leemhuis, Justyna M. Dobruchowska, Lubbert Dijkhuizen, Bauke W. Dijkstra, Tjaard Pijning |
|---|---|
| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, X-ray Crystallography |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DIRECTED EVOLUTION
CYCLODEXTRIN GLYCOSYLTRANSFERASE Cyclodextrin glycosyltransferase reuteransucrase Biochemistry Catalysis AMINO-ACID-RESIDUES ALPHA-AMYLASE FAMILY Glucansucrase Glycoside hydrolase glycoside hydrolase MOLECULAR CHARACTERIZATION CHEMOENZYMATIC SYNTHESIS alternansucrase chemistry.chemical_classification STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASES biology dextransucrase Mutagenesis Glycosidic bond protein engineering Protein engineering Directed evolution Alternansucrase lactic acid bacteria GTF-I chemistry biology.protein LEUCONOSTOC-MESENTEROIDES LACTOBACILLUS-REUTERI Biotechnology |
| Zdroj: | Biocatalysis and Biotransformation, 30(3), 366-376 |
| ISSN: | 1024-2422 |
| Popis: | Many lactic acid bacteria produce extracellular alpha-glucan polysaccharides using a glucansucrase and sucrose as glucose donor. The structure and the physicochemical properties of the alpha-glucans produced are determined by the nature of the glucansucrase. Typically, the alpha-glucans contain two types of alpha-glycosidic linkages, for example, (alpha 1-2), (alpha 1-3), (alpha 1-4) or (alpha 1-6), which may be randomly or regularly distributed. Usually, the alpha-glucan chains are also branched, which gives rise to an additional level of complexity. Even though the first crystal structure was reported in 2010, our current understanding of the structure-function relationships of glucansucrases is not advanced enough to predict the alpha-glucan specificity from the sequence alone. Nevertheless, based on sequence alignments and site-directed mutagenesis, a few amino acid residues have been identified as being important for the glycosidic bond specificity of glucansucrases. A new development in GH70 research was the identification of a cluster of alpha-glucan disproportionating enzymes. Here, we discuss the current insights into the structure-function relationships of GH70 enzymes in the light of the recently determined crystal structure of glucansucrases. |
| Databáze: | OpenAIRE |
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