Novel Clove Essential Oil Nanoemulgel Tailored by Taguchi’s Model and Scaffold-Based Nanofibers: Phytopharmaceuticals with Promising Potential as Cyclooxygenase-2 Inhibitors in External Inflammation

Autor: Reham Mokhtar Aman, M. M. Meshali, Irhan Ibrahim Abu Hashim
Rok vydání: 2020
Předmět:
Male
nanoemulgel
Administration
Topical
Syzygium
Phytochemicals
Nanofibers
Pharmaceutical Science
biopolymers
02 engineering and technology
01 natural sciences
Polyvinyl alcohol
law.invention
Chitosan
Mice
chemistry.chemical_compound
law
Gum acacia
Spectroscopy
Fourier Transform Infrared
Drug Discovery
anti-inflammatory activity
Original Research
Tissue Scaffolds
biology
General Medicine
021001 nanoscience & nanotechnology
Controlled release
Emulsions
0210 nano-technology
Skin Absorption
Biophysics
Bioengineering
010402 general chemistry
Permeability
Biomaterials
clove essential oil
In vivo
Animals
Rats
Wistar
Essential oil
Inflammation
Chromatography
Guar gum
Cyclooxygenase 2 Inhibitors
Organic Chemistry
Skin Irritancy Tests
biology.organism_classification
Taguchi’s model
0104 chemical sciences
Kinetics
chemistry
Clove Oil
Nanofiber
Zdroj: International Journal of Nanomedicine
ISSN: 1178-2013
Popis: Purpose Clove essential oil is a phytochemical possessing a vast array of biological activities. Nevertheless, fabricating nano topical delivery systems targeted to augment the anti-inflammatory activity of the oil has not been investigated so far. Accordingly, in this study, controlled release nanoparticulate systems, namely nanoemulgel and nanofibers (NFs), of the oil were developed to achieve such goal. Methods The nanoemulsion was incorporated in the hydrogel matrix of mixed biopolymers - chitosan, guar gum and gum acacia - to formulate nanoemulsion-based nanoemulgel. Taguchi's model was adopted to evaluate the effect of independently controlled parameters, namely, the concentration of chitosan (X1), guar gum (X2), and gum acacia (X3) on different dependently measured parameters. Additionally, the nanoemulsion-based NFs were prepared by the electrospinning technique using polyvinyl alcohol (PVA) polymer. Extensive in vitro, ex vivo and in vivo evaluations of the aforementioned formulae were conducted. Results Both Fourier transform-infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) established the complete dispersion of the nanoemulsion in the polymeric matrices of the prepared nanoemulgel and NFs. The ex vivo skin permeation data of clove essential oil from the prepared formulations showed that NFs can sustain its penetration through the skin comparably with nanoemulgel. Topical treatment with NFs (once application) and nanoemulgel (twice application) evoked a marvelous in vivo anti-inflammatory activity against croton oil-induced mouse skin inflammation model when compared with pure clove essential oil along with relatively higher efficacy of medicated NFs than that of medicated nanoemulgel. Such prominent anti-inflammatory activity was affirmed by histopathological and immunohistochemical examinations. Conclusion These results indicated that nanoemulsion-based nanoemulgel and nanoemulsion-based NFs could be introduced to the phytomedicine field as promising topical delivery systems for effective treatment of inflammatory diseases instead of nonsteroidal anti-inflammatory drugs that possess adverse effects.
Databáze: OpenAIRE
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