CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

Autor: Seiji Nakamura, Keita Mochizuki, Akira Chinju, A. S.M.Rafiul Haque, Akihiko Tanaka, Jun Nosuke Hayashida, Takashi Maehara, Taiki Sakamoto, Naoki Kaneko, Keigo Kubota, Ryusuke Munemura, Shintaro Kawano, Mizuki Sakamoto, Noriko Ishiguro, Masafumi Moriyama, Tamotsu Kiyoshima
Rok vydání: 2019
Předmět:
Male
Biopsy
lcsh:Medicine
Tumor Microenvironment
skin and connective tissue diseases
lcsh:Science
Aged
80 and over
Multidisciplinary
medicine.diagnostic_test
integumentary system
Chemistry
Oral cancer detection
Scavenger Receptors
Class A
Middle Aged
Immunohistochemistry
Gene Expression Regulation
Neoplastic
Carcinoma
Squamous Cell
Disease Progression
Female
Mouth Neoplasms
Mannose Receptor
hormones
hormone substitutes
and hormone antagonists
Cancer microenvironment
Adult
Antigens
Differentiation
Myelomonocytic
Receptors
Cell Surface
Article
Flow cytometry
Immune system
stomatognathic system
Antigens
CD
medicine
Carcinoma
Humans
Lectins
C-Type
Neoplasm Invasiveness
Aged
Cell Proliferation
Tumor microenvironment
Epidermal Growth Factor
Cell growth
Macrophages
lcsh:R
medicine.disease
stomatognathic diseases
Mannose-Binding Lectins
Tumor progression
Cancer research
lcsh:Q
CD163
Zdroj: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
ISSN: 2045-2322
DOI: 10.1038/s41598-019-51149-1
Popis: Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators and preferentially express CD163, CD204, and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Here we investigated the expression and function of TAM subsets in OSCC, especially in cancer cell proliferation. Biopsy sample from 44 patients with OSCC were examined for the expression of TAM markers and EGF by immunohistochemistry. EGF production of TAM subsets isolated from OSCC patients was assessed by flow cytometry. We also examined the effect of conditioned medium from TAM subsets on the proliferation of OSCC cells. CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis found that CD206+ TAMs strongly produced EGF compared with CD163+ and CD204+ TAMs. Cell proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with worse clinical prognosis. Our results revealed differences in localization and EGF production among these TAM subsets. CD206+ TAMs might play a critical role in the proliferation of OSCC via EGF production.
Databáze: OpenAIRE
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