NAD Biosynthesis
| Autor: | Oleg V. Kurnasov, Vasiliy N. Goral, Shubha Anantha, Svetlana Gerdes, Andrei L. Osterman, Tadhg P. Begley, Keri L. Colabroy |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
0303 health sciences biology 030306 microbiology Clinical Biochemistry Tryptophan General Medicine biology.organism_classification Quinolinate Genome Biochemistry 03 medical and health sciences chemistry.chemical_compound chemistry Drug Discovery Molecular Medicine NAD+ kinase Gene Molecular Biology Bacteria 030304 developmental biology Quinolinic acid Dihydroxyacetone phosphate |
| Zdroj: | Chemistry & Biology. 10(12):1195-1204 |
| ISSN: | 1074-5521 |
| DOI: | 10.1016/j.chembiol.2003.11.011 |
| Popis: | Previous studies have demonstrated two different biosynthetic pathways to quinolinate, the universal de novo precursor to the pyridine ring of NAD. In prokaryotes, quinolinate is formed from aspartate and dihydroxyacetone phosphate; in eukaryotes, it is formed from tryptophan. It has been generally believed that the tryptophan to quinolinic acid biosynthetic pathway is unique to eukaryotes; however, this paper describes the use of comparative genome analysis to identify likely candidates for all five genes involved in the tryptophan to quinolinic acid pathway in several bacteria. Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays. |
| Databáze: | OpenAIRE |
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