Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study
| Autor: | Jean-Claude Imber, Yacine El Tawil, Nikola Saulacic, Anton Sculean, Hiroki Katagiri, Masako Fujioka-Kobayashi, Niklaus P. Lang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
collagen
Bone density animal experiment histological analysis Medicine (miscellaneous) Calvaria 610 Medicine & health 02 engineering and technology micro-CT Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine In vivo medicine Bone regeneration lcsh:QH301-705.5 Chemistry Biomaterial Soft tissue hydroxyapatite Histology 030206 dentistry 021001 nanoscience & nanotechnology Membrane medicine.anatomical_structure lcsh:Biology (General) critical size defect 0210 nano-technology Biomedical engineering |
| Zdroj: | Biomedicines Volume 9 Issue 2 Biomedicines, Vol 9, Iss 143, p 143 (2021) Katagiri, Hiroki; El Tawil, Yacine; Lang, Niklaus P.; Imber, Jean-Claude; Sculean, Anton; Kobayashi, Masako; Saulacic, Nikola (2021). Collagen-Based Matrices for Osteoconduction: A Preclinical In Vivo Study. Biomedicines, 9(2) MDPI 10.3390/biomedicines9020143 |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines9020143 |
| Popis: | The aim of this study was to evaluate the influence of additional hydroxyapatite (HA) in collagen-based matrices (CM) and membrane placement on bone formation in calvarial defects. Critical size defects in the calvaria of 16 New Zealand White Rabbits were randomly treated with CM or mineralized collagen-based matrices (mCM). Half of the sites were covered with a collagen membrane. Animals were euthanized after 12 weeks of healing. The samples were studied by micro-CT and histology. Newly formed lamellar bone was observed in all samples at the periphery of the defect. In the central areas, however, new bone composed of both woven and lamellar bone was embedded in the soft tissue. Samples treated with mCM showed more residual biomaterial and induced more small bony islands in the central areas of the defects than samples with CM. Nevertheless, a complete defect closure was not observed in any of the samples at 12 weeks. Membrane placement resulted in a decrease in bone density and height. Significant differences between the groups were revealed only between CM groups with and without membrane coverage for bone height in the central area of the defect. Neither mineralization of CM nor membrane placement improved the osteogenic capacity in this particular defect. Nevertheless, mineralisation influenced bone density without a membrane placement and bone volume underneath a membrane. CM may be used as a scaffold in bone regeneration procedures, without the need of a membrane coverage. Further preclinical studies are warrant to optimise the potential of mCM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|