Visualization of thalamic calcium influx with quantitative susceptibility mapping as a potential imaging biomarker for repeated mild traumatic brain injury
Autor: | David J. Poulsen, Jenni Kyyriäinen, Kaitlynn Donahue, Kathryn Toffolo, Ferdinand Schweser, Austin Poulsen, Asla Pitkänen, Benett Levy, Marilena Preda |
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Rok vydání: | 2019 |
Předmět: |
Male
Pathology medicine.medical_specialty Imaging biomarker Traumatic brain injury Cognitive Neuroscience Thalamus Neuroimaging Article 050105 experimental psychology 03 medical and health sciences 0302 clinical medicine Skull fracture In vivo medicine Animals 0501 psychology and cognitive sciences Rats Wistar Brain Concussion business.industry 05 social sciences Calcinosis Quantitative susceptibility mapping medicine.disease Magnetic Resonance Imaging Pathophysiology Rats nervous system diseases Disease Models Animal nervous system Neurology Biomarker (medicine) Calcium business Biomarkers 030217 neurology & neurosurgery |
Zdroj: | Neuroimage |
ISSN: | 1053-8119 |
Popis: | A key event in the pathophysiology of traumatic brain injury (TBI) is the influx of substantial amounts of Ca2+ into neurons, particularly in the thalamus. Detection of this calcium influx in vivo would provide a window into the biochemical mechanisms of TBI with potentially significant clinical implications. In the present work, our central hypothesis was that the Ca2+ influx could be imaged in vivo with the relatively recent MRI technique of quantitative susceptibility mapping (QSM). Wistar rats were divided into five groups: naive controls, sham-operated experimental controls, single mild TBI, repeated mild TBI, and single severe TBI. We employed the lateral fluid percussion injury (FPI) model, which replicates clinical TBI without skull fracture, performed 9.4 Tesla MRI with a 3D multi-echo gradient-echo sequence at weeks 1 and 4 post-injury, computed susceptibility maps using V-SHARP and the QUASAR-HEIDI technique, and performed histology. Sham, experimental controls animals, and injured animals did not demonstrate calcifications at 1 week after the injury. At week 4, calcifications were found in the ipsilateral thalamus of 25–50% of animals after a single TBI and 83% of animals after repeated mild TBI. The location and appearance of calcifications on stained sections was consistent with the appearance on the in vivo susceptibility maps (correlation of volumes: r = 0.7). Our findings suggest that persistent calcium deposits represent a primary pathology of repeated injury and that FPI-QSM has the potential to become a sensitive tool for studying pathophysiology related to mild TBI in vivo. |
Databáze: | OpenAIRE |
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