Emodin Induces Death in Human Cervical Cancer Cells Through Mitotic Catastrophe
| Autor: | Anna Kopacz-Bednarska, Wojciech Trybus, Ewa Trybus, T. Krol, Anna Stachurska, Grzegorz Król |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Emodin Mitotic index Cell Survival Neutrophils Mitosis Uterine Cervical Neoplasms Anthraquinones Apoptosis HeLa 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Propidium iodide Mitotic catastrophe Cytoskeleton Cell Proliferation Micronucleus Tests Microscopy Confocal biology Chemistry Cell Cycle General Medicine Cell cycle biology.organism_classification Molecular biology Actins Oncology 030220 oncology & carcinogenesis Female Fluorescein-5-isothiocyanate HeLa Cells Propidium |
| Zdroj: | Anticancer Research. 39:679-686 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13163 |
| Popis: | Background Anthraquinones, including emodin, are compounds with numerous pharmacological properties, including anticancer properties. The aim of this study experiment was to examine the effect of emodin, a natural compound present in the roots and rhizomes of Rheum palmatum, on the induction of mitotic catastrophe in cervical cancer cells. Material and methods HeLa celIs were treated with different emodin concentrations for 48 h, and cell growth was measured with 3-(4-,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolyl. The cell-cycle distribution and the level of apoptosis were determined by means of flow cytometry, using annexin V-fluorescein isothiocyanate staining and propidium iodide. Morphological changes in the mitotic apparatus were evaluated using optical and confocal microscopy techniques. Results Emodin induced an increase in the number of polymorphonuclear cells, giant cells, cells with micronuclei, cells with abnormal mitosis and damaged spindle. The reorganization of F-actin depended on the concentration of emodin. With the increase in emodin concentration, inhibition of mitotic activity was demonstrated, which was manifested by a decrease in the mitotic index, mainly in metaphase of the mitotic process and an increase in the number of cells inhibited in the G2/M phase. At the same time, an increase in the number of apoptotic cells was found. Conclusion Emodin leads to death of cervical cancer cells by induction of a mitotic catastrophe. |
| Databáze: | OpenAIRE |
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