New method for determination of fecal sterols in urine using non-chlorinated solvents
| Autor: | Elisabet Börjesson, Rhys Leeming, Annika Sundin, Lennart Torstensson |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Methyl Ethers
Detection limit Chromatography Sewage Silylation Chemistry Methanol General Chemistry Urine Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Sterol Cholestanol Coprostanol Feces Sterols chemistry.chemical_compound Liquid–liquid extraction Solvents polycyclic compounds Humans Gas chromatography Saponification |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 713:438-442 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(98)00197-2 |
| Popis: | A new method has been developed to determine a number of sterols in urine using non-chlorinated solvents, namely methyl tert .-butyl ether and methanol (the MTB method). The method involves liquid–liquid extraction, saponification, reextraction, silylation and final identification and quantification by GC–FID. The sterols determined were coprostanol, epicoprostanol, cholesterol and dihydrocholesterol. 5α-Cholestane was used as internal standard. The limit of detection for the sterols in this experiment was 2 μg l −1 urine. Recovery of coprostanol over the range 5–100 μg l −1 urine by this method was between 80 and 92%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|