Interactions of proteoliposomes from serogroup B Neisseria meningitidis with bone marrow-derived dendritic cells and macrophages: adjuvant effects and antigen delivery

Autor: Sanja Ugrinovic, Nathalie Ménager, Oliver Pérez, Gustavo Bracho, Tamara Rodríguez, Pietro Mastroeni
Rok vydání: 2005
Předmět:
CD4-Positive T-Lymphocytes
Ovalbumin
Proteolipids
Genes
MHC Class II
Antigen-Presenting Cells
Bone Marrow Cells
Enzyme-Linked Immunosorbent Assay
Meningococcal Vaccines
Receptors
Cell Surface
chemical and pharmacologic phenomena
CD8-Positive T-Lymphocytes
Hybrid Cells
Neisseria meningitidis
Serogroup B
Biology
Cell Line
Microbiology
Mice
Adjuvants
Immunologic
Antigen
Animals
Macrophage
CD86
Antigen Presentation
Antigens
Bacterial
Mice
Inbred C3H
CD40
General Veterinary
General Immunology and Microbiology
Macrophages
Public Health
Environmental and Occupational Health
hemic and immune systems
Dendritic Cells
Dendritic cell
Flow Cytometry
Molecular biology
Up-Regulation
Mice
Inbred C57BL
Toll-Like Receptor 4
Infectious Diseases
Interleukin 12
biology.protein
Cytokines
Molecular Medicine
Female
CD80
Zdroj: Vaccine. 23:1312-1321
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2004.07.049
Popis: Exposure to proteoliposomes from serogroup B Neisseria meningitidis (PL) induced up-regulation of MHC-II, MHC-I, CD40, CD80 and CD86 expression on the surface of murine bone marrow-derived dendritic cells (DC). CD40, CD80 and CD86 were up-regulated on bone marrow-derived macrophages (MΦ) upon stimulation with PL. Both DC and MΦ released TNFα, but only DC produced IL12(p70) in response to PL. A small increase in the expression of MHC-II, CD40 and CD86, as well as production of IL12(p70), was observed on the cell surface of DC, but not MΦ from LPS-non-responder C3H/HeJ after exposure to PL. DC, but not MΦ, incubated with PL containing ovalbumin (PL-OVA) presented OVA-specific peptides to CD4 + and CD8 + OVA-specific T-cell hybridomas. These data clearly indicate that PL exert an immunomodulatory effect on DC and MΦ, with some contribution of non-LPS components besides the main role of LPS. The work also shows the potential of PL as a general system to deliver antigens to DC for presentation to CD4 + and CD8 + T-cells.
Databáze: OpenAIRE
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