Genome Analysis and Characterisation of the Exopolysaccharide Produced by Bifidobacterium longum subsp. longum 35624™

Autor: Marita Gleinser, Elisabeth Svehla, Francesca Bottacini, Andreas Hofinger, Mary O'Connell Motherway, Sinead C. Leahy, Elisa Schiavi, Cezmi A. Akdis, David Groeger, Markus Windwarder, Stephan Plattner, Noelia Rodriguez Perez, Evelyn M. Molloy, Liam O'Mahony, Paul Kosma, Jennifer Roper, Douwe van Sinderen, Selena Healy, Ray Grant, Jun Xu, Amy O’Callaghan, Friedrich Altmann
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Exopolysaccharides
Bifidobacterium longum
Glycobiology
lcsh:Medicine
Gene prediction
Spectrum analysis techniques
Biochemistry
Genome
chemistry.chemical_compound
fluids and secretions
Gene cluster
lcsh:Science
Bifidobacterium
Multidisciplinary
Strain (chemistry)
biology
Organic Compounds
Physics
Monosaccharides
food and beverages
Genomics
Chemistry
Physical Sciences
Protons
Research Article
030106 microbiology
Carbohydrates
03 medical and health sciences
NMR spectroscopy
Polysaccharides
Genetics
Gene Prediction
Nuclear Physics
Nucleons
Comparative genomics
Whole genome sequencing
Bacteria
Gut Bacteria
Organic Chemistry
lcsh:R
Organisms
Chemical Compounds
Biology and Life Sciences
Computational Biology
Comparative Genomics
Genome Analysis
biology.organism_classification
Research and analysis methods
030104 developmental biology
chemistry
Galactose
lcsh:Q
Zdroj: PLoS ONE, Vol 11, Iss 9, p e0162983 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: The Bifibobacterium longum subsp. longum 35624™ strain (formerly named Bifidobacterium longum subsp. infantis) is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. This paper presents (a) the genome sequence of the organism allowing the assignment to its correct subspeciation longum; (b) a comparative genome assessment with other B. longum strains and (c) the molecular structure of the 35624 exopolysaccharide (EPS624). Comparative genome analysis of the 35624 strain with other B. longum strains determined that the sub-speciation of the strain is longum and revealed the presence of a 35624-specific gene cluster, predicted to encode the biosynthetic machinery for EPS624. Following isolation and acid treatment of the EPS, its chemical structure was determined using gas and liquid chromatography for sugar constituent and linkage analysis, electrospray and matrix assisted laser desorption ionization mass spectrometry for sequencing and NMR. The EPS consists of a branched hexasaccharide repeating unit containing two galactose and two glucose moieties, galacturonic acid and the unusual sugar 6-deoxy-L-talose. These data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide.
Databáze: OpenAIRE
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