Luteinizing hormone modulates intracellular calcium, protein tyrosine phosphorylation and motility during human sperm capacitation
Autor: | Fernando Larrea, Claudia L. Treviño, Aideé Saray López-Torres, María Elena González-González, Mayel Chirinos, Esperanza Mata-Martínez |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty Acrosome reaction Biophysics Motility Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Capacitation Internal medicine medicine Humans Calcium Signaling Phosphorylation Phosphotyrosine Molecular Biology Sperm motility 030219 obstetrics & reproductive medicine Hyperactivation Acrosome Reaction Tyrosine phosphorylation Cell Biology Luteinizing Hormone Sperm 030104 developmental biology Endocrinology chemistry Sperm Motility Calcium Female Luteinizing hormone Sperm Capacitation |
Zdroj: | Biochemical and Biophysical Research Communications. 483:834-839 |
ISSN: | 0006-291X |
DOI: | 10.1016/j.bbrc.2017.01.010 |
Popis: | In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation are modulated by their interaction with molecules present in the female reproductive tract. During the woman fertile window, some reproductive hormones reach their maximum concentrations in serum, such as the luteinizing hormone (LH). Since spermatozoa preparing to fertilize may be exposed to LH, the purpose of this work was to study the effects of this hormone on intracellular Ca2+ concentrations ([Ca2+]i), protein tyrosine phosphorylation, sperm motility and acrosome reaction under capacitating conditions. The results showed that LH increases the duration and amplitude of Ca2+ oscillations. Furthermore, motility analysis indicated that LH decreases rapid progressive motility and that sperm hyperactivation as well as several kinetic parameters augment in the presence of 0.5 and 1 μg/ml of the hormone. In addition, these two hormone concentrations also consistently promoted protein tyrosine phosphorylation. However, no effects on acrosome reaction were observed. In conclusion, the evidence indicates that LH modulates several sperm function variables involved in capacitation, suggesting that may have an important and unexplored role during human fertilization. |
Databáze: | OpenAIRE |
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