The comet assay differentiates efficiently and rapidly between genotoxins and cytotoxins in quiescent cells
| Autor: | Paula Daza, Francisco Javier Moreno, José María Maestre Torreblanca |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Antimetabolites Antineoplastic Lymphocyte Cell Antineoplastic Agents Biology chemistry.chemical_compound Leukocytes medicine Humans Enzyme Inhibitors Cytotoxicity Deoxyadenosines Cordycepin DNA Cell Biology General Medicine Molecular biology In vitro Comet assay medicine.anatomical_structure chemistry Puromycin Dactinomycin Camptothecin Comet Assay Floxuridine Mutagens medicine.drug |
| Zdroj: | Cell Biology International. 28:497-502 |
| ISSN: | 1065-6995 |
| Popis: | Our main aim was to establish the efficiency of the single cell electrophoresis technique for differentiating between drugs that bind DNA and those that do not. The alkaline comet assay was used to test the responses of human leukocytes (quiescent cells) to damage induced by reportedly genotoxic and reportedly cytotoxic agents. Incubation of G0 leukocytes for 1 h with the genotoxic agents camptothecin and actinomycin C provoked DNA migration, observed as comet figures. On the other hand, when cells were treated with the cytotoxic agents cordycepin, fluorodeoxyuridine and puromycin, the leukocyte nuclei were indistinguishable from those of untreated cells. In addition, we have developed a rapid method using non-proliferating cells that requires neither culture nor lymphocyte isolation. This method promises to be useful as a rapid in vitro screening assay. |
| Databáze: | OpenAIRE |
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