Lactobacillus paracasei KW3110 Suppresses Inflammatory Stress-Induced Premature Cellular Senescence of Human Retinal Pigment Epithelium Cells and Reduces Ocular Disorders in Healthy Humans
| Autor: | Yuji Morita, Konomi Ohshio, Sayuri Yamada, Takahiro Yamazaki, Miho Sugamata, Takahiro Yamada, Hiroaki Suzuki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty retina Inflammation eye fatigue Catalysis Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Immune system Downregulation and upregulation Internal medicine medicine cellular senescence Physical and Theoretical Chemistry Molecular Biology Cell damage lcsh:QH301-705.5 Spectroscopy Retina Retinal pigment epithelium 030102 biochemistry & molecular biology Tight junction business.industry Organic Chemistry General Medicine medicine.disease Computer Science Applications lactic acid bacteria 030104 developmental biology medicine.anatomical_structure Endocrinology lcsh:Biology (General) lcsh:QD1-999 probiotics inflammation Eye disorder sense organs medicine.symptom business |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 5091, p 5091 (2020) Volume 21 Issue 14 |
| ISSN: | 1422-0067 |
| Popis: | Lactobacillus paracasei KW3110 (KW3110) has anti-inflammatory effects and mitigates retinal pigment epithelium (RPE) cell damage caused by blue-light exposure. We investigated whether KW3110 suppresses chronic inflammatory stress-induced RPE cell damage by modulating immune cell activity and whether it improves ocular disorders in healthy humans. First, we showed that KW3110 treatment of mouse macrophages (J774A.1) produced significantly higher levels of interleukin-10 as compared with other lactic acid bacterium strains (all p < 0.01). Transferring supernatant from KW3110- and E. coli 0111:B4 strain and adenosine 5&prime triphosphate (LPS/ATP)-stimulated J774A.1 cells to human retinal pigment epithelium (ARPE-19) cells suppressed senescence-associated phenotypes, including proliferation arrest, abnormal appearance, cell cycle arrest, and upregulation of cytokines, and also suppressed expression of tight junction molecule claudin-1. A randomized, double-blind, placebo-controlled parallel-group study of healthy subjects (n = 88 35 to below 50 years) ingesting placebo or KW3110-containing supplements for 8 weeks showed that changes in critical flicker frequency, an indicator of eye fatigue, from the week-0 value were significantly larger in the KW3110 group at weeks 4 (p = 0.040) and 8 (p = 0.036). These results suggest that KW3110 protects ARPE-19 cells against premature senescence and aberrant expression of tight junction molecules caused by chronic inflammatory stress, and may improve chronic eye disorders including eye fatigue. |
| Databáze: | OpenAIRE |
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