Effect of basal lamina of ovarian follicle on T- and L-type Ca2+currents in differentiated granulosa cells
| Autor: | Stanley G. Rane, Wuxuan Qin, Elikplimi K. Asem |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Time Factors Calcium Channels L-Type Physiology Endocrinology Diabetes and Metabolism Preservation Biological Ovary Electric Capacitance Basement Membrane Extracellular matrix Calcium Channels T-Type Type IV collagen Ovarian Follicle Laminin Physiology (medical) Internal medicine medicine Animals Patch clamp Cycloheximide Ovarian follicle Cell Size Nucleic Acid Synthesis Inhibitors Protein Synthesis Inhibitors Basement membrane Granulosa Cells biology Electric Conductivity medicine.anatomical_structure Endocrinology Dactinomycin biology.protein Female Basal lamina Chickens |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 282:E184-E196 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.2002.282.1.e184 |
| Popis: | Patch clamp experiments were conducted to study the effects of basal lamina (basement membrane) of chicken ovarian follicle on membrane Ca2+currents in differentiated chicken granulosa cells in a homologous system. The whole cell patch clamp technique was used to simultaneously monitor membrane capacitance (an indirect measure of total cell surface area) and currents flowing through voltage-dependent Ca2+channels (using Ba2+as the charge carrier). Membrane capacitance was smaller in cells incubated on intact basal lamina than in control cells (incubated on tissue culture-treated plastic substratum). Granulosa cells expressed both T- and L-type Ca2+currents, and the amplitudes of the currents in cells incubated on intact basal lamina were significantly lower than those of control cells. Also, granulosa cells incubated on intact basal lamina were found to have significantly lower T- or L-type Ca2+current densities than control cells. Intact basal lamina that had been stored for 12 mo produced effects on T- and L-type Ca2+currents similar to those caused by freshly isolated basal lamina. The basal lamina was solubilized completely in one step and used to coat glass coverslips (uncoated glass coverslips served as controls). Granulosa cells incubated on coverslips precoated with solubilized basal lamina assumed spherical shape similar to those incubated on intact basal lamina. Similar to the observations made for intact basal lamina, the solubilized basal lamina suppressed T- and L-type Ca2+currents in the differentiated granulosa cells. Moreover, fibronectin, laminin, and type IV collagen, obtained from commercial sources, attenuated T- and L-type Ca2+currents in the differentiated granulosa cells. This interplay between basal lamina and Ca2+currents may be one mechanism that subserves the effects of the matrix material on metabolic functions of granulosa cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|