Effects of tetrahydrobiopterin on cerebral infarction after transient focal ischemia in rats
| Autor: | Jie-Sheng Zheng, Ling-Ling Tang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_treatment Intraperitoneal injection Focal ischemia Nitric Oxide Brain Ischemia Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Animals Medicine Middle cerebral artery occlusion biology business.industry Cerebral infarction Infarction Middle Cerebral Artery Cerebral Infarction General Medicine Tetrahydrobiopterin medicine.disease Infarct size Biopterin Rats Nitric oxide synthase Disease Models Animal Neurology chemistry Ischemic Attack Transient Reperfusion Injury Anesthesia biology.protein Neurology (clinical) Nitric Oxide Synthase business Signal Transduction medicine.drug |
| Zdroj: | Neurological Research. 33:1064-1067 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1179/016164110x12700393823651 |
| Popis: | Objectives: The present study investigated the effects of tetrahydrobiopterin (BH4) on cerebral infarction after transient focal ischemia in rats. Methods: Focal ischemia (1·5 hours) was created in male Sprague-Dawley rats (250-280 g) by middle cerebral artery occlusion. Some rats were treated with 20 mg/kg tetrahydrobiopterin by intraperitoneal injection 30 minutes before reperfusion. At 2, 6, and 12 hours of reperfusion, the brains were harvested for the nitric oxide synthase (NOS) activity and nitric oxide (NO) level assays. At 12 hours of reperfusion, the brains were harvested for infarct size measurement. Results: NOS activity and NO level were all augmented after reperfusion. BH4 treatment significantly further increased NOS activity and NO level. Cerebral infarct size was significantly bigger in BH4 treatment group compared to that in no treatment group. Conclusions: The data indicate that BH4 enhances cerebral infarction after transient focal ischemia in rats, through NOS and NO pathway. |
| Databáze: | OpenAIRE |
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