Low dose short duration pulsed electromagnetic field effects on cultured human chondrocytes: An experimental study
| Autor: | Selvam Anbarasan, Ulaganathan Baraneedharan, Solomon F. D. Paul, Subramoniam Rangaswami, Emmanuel Bhaskar, Harpreet Kaur |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Post exposure Joint replacement medicine.medical_treatment Osteoarthritis pulsed electromagnetic field Andrology Extracellular matrix 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery Human chondrocytes medicine Orthopedics and Sports Medicine Short duration mesh:cartilage business.industry Cartilage mesh:chondrocytes Low dose mesh:articular medicine.disease lcsh:RD701-811 osteoarthritis 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Joint disorder Original Article mesh:Osteoarthritis business mesh:electromagnetic fields |
| Zdroj: | Indian Journal of Orthopaedics Indian Journal of Orthopaedics, Vol 50, Iss 1, Pp 87-93 (2016) |
| ISSN: | 1998-3727 0019-5413 |
| Popis: | Background: Pulsed electromagnetic field (PEMF) is used to treat bone and joint disorders for over 30 years. Recent studies demonstrate a significant effect of PEMF on bone and cartilage proliferation, differentiation, synthesis of extracellular matrix (ECM) and production of growth factors. The aim of this study is to assess if PEMF of low frequency, ultralow field strength and short time exposure have beneficial effects on in-vitro cultured human chondrocytes. Materials and Methods: Primary human chondrocytes cultures were established using articular cartilage obtained from knee joint during joint replacement surgery. Post characterization, the cells were exposed to PEMF at frequencies ranging from 0.1 to 10 Hz and field intensities ranging from 0.65 to 1.95 μT for 60 min/day for 3 consecutive days to analyze the viability, ECM component synthesis, proliferation and morphology related changes post exposure. Association between exposure doses and cellular effects were analyzed with paired't' test. Results: In-vitro PEMF exposure of 0.1 Hz frequency, 1.95 μT and duration of 60 min/day for 3 consecutive days produced the most favorable response on chondrocytes viability (P < 0.001), ECM component production (P < 0.001) and multiplication. Exposure of identical chondrocyte cultures to PEMFs of 0.65 μT field intensity at 1 Hz frequency resulted in less significant response. Exposure to 1.3 μT PEMFs at 10 Hz frequency does not show any significant effects in different analytical parameters. Conclusions: Short duration PEMF exposure may represent a new therapy for patients with Osteoarthritis (OA). |
| Databáze: | OpenAIRE |
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