New feruloyl esterases to access phenolic acids from grass biomass

Autor: Stephan Grosse, Kofi Abokitse, Peter C. K. Lau, Hannes Leisch, Meiqun Wu
Rok vydání: 2011
Předmět:
antioxidant
biocatalyst
Alkalies
medicine.disease_cause
Furfural
Applied Microbiology and Biotechnology
Biochemistry
Substrate Specificity
Ferulic acid
chemistry.chemical_compound
lignocellulose
Enzyme Stability
Hydroxybenzoates
Biomass
Myxococcales
triticale
Conserved Sequence
Triticum
biorefinery
biology
phenolic acid
food and beverages
General Medicine
Triticale
α/ß-hydrolase
Hydrogen-Ion Concentration
myxobacteria
Electrophoresis
Polyacrylamide Gel
Biotechnology
platform chemicals
Coumaric Acids
Molecular Sequence Data
Bioengineering
Poaceae
Myxobacteria
genome mining
medicine
Amino Acid Sequence
Molecular Biology
Escherichia coli
Sorangium cellulosum
Enzyme Assays
Bran
Phenolic acid
biology.organism_classification
Molecular Weight
Kinetics
chemistry
Carboxylic Ester Hydrolases
Sequence Alignment
Zdroj: Applied biochemistry and biotechnology. 168(1)
ISSN: 1559-0291
Popis: In the Sorangium cellulosum strain So ce56 genome, two putative esterase-encoding genes (loci sce1896 and sce8927) were cloned, expressed in Escherichia coli, and the resulting enzymes (designated ScFAE1 and ScFAE2) were used to assess the possible release of ferulic acid (FA) from triticale and wheat brans, and an aqueous fraction of steam-exploded wheat straw. The two polypeptides, sharing only 30% sequence identity, exhibit a typical catalytic Ser-Asp-His triad, a characteristic of α/β-hydrolase fold proteins. Both ScFAE1 (35 kDa) and ScFAE2 (34 kDa) were purified to apparent homogeneity and comparison of their kinetic parameters indicated an apparent higher affinity of ScFAE2 than ScFAE1 towards the various feruloyl substrates. This property was reflected by the observation that ScFAE2 was capable of yielding up to 85% of FA from destarched triticale bran. In the steam-exploded wheat sample, more than 85% yield of FA or p-coumaric acid was also effected by ScFAE2 without the decomposition of valuable chemical such as furfural. The two cloned FAEs represent the first of myxobacterial origin to be characterized and they are classified as new members of the type D family of FAEs.
Databáze: OpenAIRE
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