c-Src promotes the growth and tumorigenesis of hepatocellular carcinoma via the Hippo signaling pathway

Autor: Xin Yang, Qingfu Qian, Jing Yang, Xiujuan Zhang, Leilei Liu, Bin Du
Rok vydání: 2021
Předmět:
Male
0301 basic medicine
Transcription
Genetic
Carcinogenesis
Apoptosis
medicine.disease_cause
030226 pharmacology & pharmacy
0302 clinical medicine
Cell Movement
General Pharmacology
Toxicology and Pharmaceutics
medicine.diagnostic_test
Liver Neoplasms
General Medicine
Cell cycle
Prognosis
Up-Regulation
Gene Expression Regulation
Neoplastic
Blot
Signal Transduction
Proto-oncogene tyrosine-protein kinase Src
Carcinoma
Hepatocellular
Proto-Oncogene Proteins pp60(c-src)
Down-Regulation
Mice
Nude
Protein Serine-Threonine Kinases
Biology
General Biochemistry
Genetics and Molecular Biology
Flow cytometry
03 medical and health sciences
Downregulation and upregulation
Cell Line
Tumor
medicine
Animals
Humans
Hippo Signaling Pathway
Neoplasm Invasiveness
RNA
Messenger
Protein Kinase Inhibitors
Adaptor Proteins
Signal Transducing
Cell Proliferation
Cell Nucleus
Hippo signaling pathway
YAP-Signaling Proteins
Cell Cycle Checkpoints
digestive system diseases
030104 developmental biology
Cancer research
Transcription Factors
Zdroj: Life Sciences. 264:118711
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2020.118711
Popis: We investigated the association between c-Src and the progression of hepatocellular carcinoma (HCC) and its underlying mechanisms. The relationship between c-Src expression and the occurrence and development of HCC was explored using GEPIA and further confirmed by western blotting analysis and real-time quantitative PCR. CCK-8, flow cytometry, Transwell, and wound-healing assays were conducted to analyze the effects of c-Src on the growth, cell cycle, apoptosis, migration, and infiltration of HCC cells. Mouse models of transplanted xenogeneic human tumors were constructed to explore the effects of c-Src on HCC tumor growth. Compared with that in adjacent normal liver tissues, the expression level of c-Src in HCC tissues was significantly increased and was negatively correlated with patient survival. These findings are consistent with those in the GEPIA database. Downregulation of c-Src expression can inhibit the growth, infiltration, and migration of HCC cells. c-Src impeded the translocation of YAP from the nucleus to the cytoplasm and promoted Yes-associated protein transcriptional activity. In vivo experiments showed that c-Src inhibition suppressed tumor growth in mice. We found that c-Src can promote the growth and tumorigenesis of HCC cells by activating the Hippo signaling pathway.
Databáze: OpenAIRE
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