National Malaria Prevalence in Cambodia: Microscopy Versus Polymerase Chain Reaction Estimates
| Autor: | Didier Menard, Jan Bruce, Jean Popovici, Duong Socheat, Seshu Babu Vinjamuri, William O. Rogers, Walter R. J. Taylor, Sylvia Meek, Frédéric Ariey, Dysoley Lek |
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| Přispěvatelé: | National Center for Parasitology, Entomology and Malaria Control [Phnom Penh, Cambodia] (CNM), National Institute of Public Health [Phnom Penh, Cambodge], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Département Parasites et Insectes vecteurs - Department of Parasites and Insect Vectors, Institut Pasteur [Paris], Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de parasitologie-mycologie [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), World Health Organization Lao People's Democratic Republic Office [Vientiane, Laos], Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), London School of Hygiene and Tropical Medicine (LSHTM), Hôpitaux Universitaires de Genève (HUG), Mahidol Oxford Tropical Medicine Research Unit (MORU), University of Oxford [Oxford]-Mahidol University [Bangkok]-Wellcome Trust, US Naval Medical Research Unit n°2, We are grateful to the study participants and for the support of the National Institute of Health Research and Development of Indonesia, and of the Eijkman Institute., Institut Pasteur [Paris] (IP), University of Oxford-Mahidol University [Bangkok]-Wellcome Trust, Wellcome Trust-Mahidol University [Bangkok]-University of Oxford [Oxford] |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Plasmodium vivax Prevalence Plasmodium malariae Polymerase Chain Reaction law.invention 0302 clinical medicine law [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Child 030212 general & internal medicine Malaria Falciparum Child Dried blood Polymerase chain reaction MESH: Plasmodium falciparum MESH: Plasmodium malariae Microscopy biology MESH: Malaria Falciparum MESH: Infant Newborn Articles MESH: Infant 3. Good health MESH: Plasmodium vivax Infectious Diseases MESH: Young Adult Child Preschool Female Cambodia medicine.medical_specialty MESH: Microscopy Adolescent Plasmodium falciparum 030231 tropical medicine MESH: Malaria Young Adult 03 medical and health sciences Virology Internal medicine parasitic diseases Malaria Vivax medicine Humans MESH: Prevalence MESH: Adolescent MESH: Humans MESH: Cambodia MESH: Child Preschool Infant Newborn Infant MESH: Malaria Vivax MESH: Polymerase Chain Reaction medicine.disease biology.organism_classification Confidence interval MESH: Male Malaria Parasitology MESH: Female |
| Zdroj: | American Journal of Tropical Medicine and Hygiene American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2016, 95 (3), pp.588-594. ⟨10.4269/ajtmh.15-0908⟩ American Journal of Tropical Medicine and Hygiene, 2016, 95 (3), pp.588-594. ⟨10.4269/ajtmh.15-0908⟩ |
| ISSN: | 0002-9637 |
| DOI: | 10.4269/ajtmh.15-0908⟩ |
| Popis: | International audience; Accurate information regarding malaria prevalence at national level is required to design and assess malaria control/elimination efforts. Although many comparisons of microscopy and polymerase chain reaction (PCR)-based methods have been conducted, there is little published literature covering such comparisons in southeast Asia especially at the national level. Both microscopic examination and PCR detection were performed on blood films and dried blood spots samples collected from 8,067 individuals enrolled in a nationwide, stratified, multistage, cluster sampling malaria prevalence survey conducted in Cambodia in 2007. The overall malaria prevalence and prevalence rates of Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae infections estimated by microscopy (N = 8,067) were 2.74% (95% confidence interval [CI]: 2.39-3.12%), 1.81% (95% CI: 1.53-2.13%), 1.14% (95% CI: 0.92-1.40%), and 0.01% (95% CI: 0.003-0.07%), respectively. The overall malaria prevalence based on PCR detection (N = 7,718) was almost 2.5-fold higher (6.31%, 95% CI: 5.76-6.89%, P < 0.00001). This difference was significantly more pronounced for P. falciparum (4.40%, 95% CI: 3.95-4.90%, P < 0.00001) compared with P. vivax (1.89%, 95% CI: 1.60-2.22%, P < 0.001) and P. malariae infections (0.22%, 95% CI: 0.13-0.35%, P < 0.0001). The significant proportion of microscopy-negative but PCR-positive individuals (289/7,491, 3.85%) suggest microscopic examination frequently underestimated malaria infections and that active case detection based on microscopy may miss a significant reservoir of infection, especially in low-transmission settings. |
| Databáze: | OpenAIRE |
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