Organotypic growth and differentiation of human mammary gland in sponge-gel matrix supported histoculture
| Autor: | Jury M. Vasiliev, Leonid Margolis, E B Polevaya, Boris Baibakov, V I Guelstein, V D Ermilova, T A Chipisheva |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Mammary gland Breast Neoplasms Biology Epithelium In vivo Culture Techniques Internal medicine Keratin medicine Humans Breast Aged chemistry.chemical_classification Myoepithelial cell Cell Biology General Medicine Middle Aged Immunohistochemistry In vitro Cell biology medicine.anatomical_structure Endocrinology chemistry Cell culture Keratins Female Developmental biology Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 30:490-495 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/bf02631321 |
| Popis: | Blocks of breast tissue obtained during radical mastectomies from 23 patients with mammary gland carcinomas were used for cultivation in native-state, gel-supported histocultures. We show that the human mammary gland can be successfully maintained in this system so that normal epithelial breast structures proliferate and undergo differentiation for several weeks and a well-developed system of ducts and lobules is formed. Using antibodies to individual keratins 17 and 8 we have shown for the first time that ducts and alveoles developing in vitro undergo differentiation into the lining epithelium and myoepithelium in the same way as mammary gland epithelium in vivo. Growth of epithelial structures in vitro is also accompanied by the development of continuous basal membrane. |
| Databáze: | OpenAIRE |
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