Tendon Regeneration with Tendon Hydrogel–Based Cell Delivery
Autor: | Elise Umansky, Michael Bachmann, Arhana Chattopadhyay, Alberto F. Lovell, Chris Crowe, James Chang, Rory McGoldrick, Mollie E. Jacobs, Anais Legrand, Michael G. Galvez |
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Rok vydání: | 2016 |
Předmět: |
musculoskeletal diseases
0301 basic medicine Cell Survival Cytological Techniques Adipose tissue Real-Time Polymerase Chain Reaction complex mixtures Hydrogel Polyethylene Glycol Dimethacrylate Tendons 03 medical and health sciences 0302 clinical medicine RNA analysis Animals Humans Regeneration Medicine Cells Cultured Skin 030222 orthopedics business.industry Stem Cells Regeneration (biology) technology industry and agriculture Biomaterial Anatomy Fibroblasts musculoskeletal system Cell delivery Rats Cell biology Tendon 030104 developmental biology medicine.anatomical_structure Adipose Tissue RNA Surgery Stem cell business |
Zdroj: | Plastic and Reconstructive Surgery. 138:617-626 |
ISSN: | 0032-1052 |
Popis: | Tendon hydrogel is a promising biomaterial for improving repair strength after tendon injury. This study compares the capacity of fibroblasts and adipose-derived stem cells to proliferate, survive, and acquire tenogenic properties when seeded into tendon hydrogel in vitro and in vivo.The effect of cell density on hydrogel contraction was measured macroscopically. To assess tenogenic properties, RNA was isolated from cells seeded in vitro in hydrogel, and tenocyte markers were quantified. To assess in vitro proliferation and survival, MTS and live-dead assays were performed. Finally, to assess the in vivo survival of cells in hydrogel, subcutaneous injections were performed on rats and in vivo imaging was performed.At 0.5 million cells/ml, both the fibroblasts and adipose-derived stem cells induced minimal hydrogel contraction compared with higher cellular concentrations. Fibroblasts and adipose-derived stem cells seeded at 0.5 million cells/ml in tendon hydrogel up-regulated several tenocyte markers after 1 week. On MTS assay, fibroblasts and adipose-derived stem cells proliferated in hydrogel at similar rates. On live-dead assay, fibroblasts survived longer than adipose-derived stem cells. With use of the in vivo imaging system and histologic evaluation, fibroblasts survived longer than adipose-derived stem cells in hydrogel in vivo.Tendon healing is mediated by the proliferation, survival, and tenogenic differentiation of cells at the site of injury. Tendon hydrogel delivering dermal fibroblasts may improve and stimulate this process compared with adipose-derived stem cells. Future studies will be needed to evaluate the effects of this hydrogel-based cell delivery on chronic tendon injuries. |
Databáze: | OpenAIRE |
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