Organic and inogranic lead inhibit neurite growth in vertebrate and invertebrate neurons in culture
| Autor: | David Shugarts, Gina M Nelson, Julie Przekwas, Gerald Audesirk |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Neurite
Lead chloride Snails Clinical Biochemistry Lymnaea stagnalis Chick Embryo Plant Science In Vitro Techniques Organometallic Compounds medicine Animals Cells Cultured Cytoskeleton Neurons Dose-Response Relationship Drug biology Cell Differentiation Embryo Cell Biology Anatomy biology.organism_classification medicine.anatomical_structure Lead Cell culture Toxicity Biophysics Neuron Developmental biology Biotechnology Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. 25:1121-1128 |
| ISSN: | 1475-2689 0883-8364 |
| DOI: | 10.1007/bf02621263 |
| Popis: | Neurons from brains of chick embryos and pond snails (Lymnaea stagnalis) were cultured for 3 to 4 d in the presence of no toxins, inorganic lead (PbCl2), or organic lead (triethyl lead chloride). In chick neurons, inorganic lead reduced the percentage of cells that grew neurites (IC50 = 270 microM total lead, approximately 70 nM free Pb2+) but did not reduce the number of neurites per cell or the mean neurite length. Triethyl lead reduced the percentage of cells that grew neurites (IC50 = 0.24 microM) and the mean neurite length (extrapolated IC50 = 3.6 microM) but did not reduce the number of neurites per cell. In Lymnaea neurons, inorganic lead reduced the percentage of cells that grew neurites (IC50 = 13 microM total lead; approximately 10 nM free Pb2+). Triethyl lead reduced the percentage of cells that grew neurites (IC50 = 0.4 microM) and exerted significant toxicity at 0.2 microM. The two forms of lead affected neurite growth in qualitatively different ways, which suggests that their mechanisms of action are different. |
| Databáze: | OpenAIRE |
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