Correction: Corrigendum: miR-218 is essential to establish motor neuron fate as a downstream effector of Isl1–Lhx3
| Autor: | Xiaolu A. Cambronne, Jae Woon Lee, Soo Kyung Lee, Stacey M. Glasgow, Ji Sun Kwon, Rongkun Shen, Heejin Nam, Seung-Hee Lee, Karen P. Thiebes, Hyong Ho Cho, Richard H. Goodman |
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| Rok vydání: | 2015 |
| Předmět: |
Neural Tube
Neurogenesis LIM-Homeodomain Proteins General Physics and Astronomy Chick Embryo Real-Time Polymerase Chain Reaction Bioinformatics General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Political science Animals Humans 030304 developmental biology Motor Neurons 0303 health sciences Multidisciplinary Effector Gene Expression Regulation Developmental Mouse Embryonic Stem Cells General Chemistry Corrigenda Up-Regulation MicroRNAs Electroporation HEK293 Cells Spinal Cord Neuroscience 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms9227 |
| Popis: | While microRNAs have emerged as an important component of gene regulatory networks, it remains unclear how microRNAs collaborate with transcription factors in the gene networks that determines neuronal cell fate. Here we show that in the developing spinal cord, the expression of miR-218 is directly upregulated by the Isl1-Lhx3 complex, which drives motor neuron fate. Inhibition of miR-218 suppresses the generation of motor neurons in both chick neural tube and mouse embryonic stem cells, suggesting that miR-218 plays a crucial role in motor neuron differentiation. Results from unbiased RISC-trap screens, in vivo reporter assays and overexpression studies indicated that miR-218 directly represses transcripts that promote developmental programs for interneurons. In addition, we found that miR-218 activity is required for Isl1-Lhx3 to effectively induce motor neurons and suppress interneuron fates. Together our results reveal an essential role of miR-218 as a downstream effector of the Isl1-Lhx3 complex in establishing motor neuron identity. |
| Databáze: | OpenAIRE |
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