Translation of BDNF-gene transcripts with short 3′ UTR in hippocampal CA1 neurons improves memory formation and enhances synaptic plasticity-relevant signaling pathways
| Autor: | Man Wang, Pietro Paolo Sanna, Yinghan Zhuang, Di Yun, Thomas Behnisch, Dongxue Li, Vez Repunte-Canonigo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Cognitive Neuroscience Experimental and Cognitive Psychology Tropomyosin receptor kinase B Hippocampal formation Mice 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Memory Neurotrophic factors Avoidance Learning Animals Learning Fear conditioning Phosphorylation 3' Untranslated Regions CA1 Region Hippocampal Neurons Neuronal Plasticity Three prime untranslated region Brain-Derived Neurotrophic Factor Fear 030104 developmental biology nervous system Protein Biosynthesis Synapses Synaptic plasticity Memory consolidation Psychology Neuroscience 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Neurobiology of Learning and Memory. 138:121-134 |
| ISSN: | 1074-7427 |
| DOI: | 10.1016/j.nlm.2016.07.004 |
| Popis: | While the brain-derived neurotrophic factor (BDNF) gene and its multiple transcripts have been recognized as a key factor for learning, but the specific involvement of BDNF translated from BDNF transcripts with short-3' untranslated region (short 3' UTR) in learning and memory requires further analysis. In this paper, we present data to show that the transduction of hippocampal CA1 neurons with AAV9-5' UTR-BDNF (short 3' UTR)-IRES-ZsGreen and the subsequent expression of BDNF enhanced the phosphorylation of synaptic plasticity relevant proteins and improved passive avoidance and object location, but not object recognition memory. In addition, BDNF improved the relearning of object location. At higher BDNF overexpression levels, the fear behavior was accompanied with a decline in the passive avoidance memory 24h post training, and with an enhanced fear conditioning performance. In addition, these animals developed spontaneous seizures. Thus, the expression of BDNF in the hippocampal CA1 region has the potential to improve fear and object location memory in wild type mouse strains when the region and expression levels of BDNF are well controlled. |
| Databáze: | OpenAIRE |
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