Core Microbiota Promotes the Development of Dental Caries
| Autor: | Mingyun Li, Biao Ren, Xuedong Zhou, Lei Cheng, Yanyan Chen, Xian Peng, Lixin Kong, Jing Chen, Jiyao Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Technology core microbiota QH301-705.5 QC1-999 030106 microbiology Streptococcus mutans Veillonella parvula Microbiology 03 medical and health sciences 0302 clinical medicine stomatognathic system In vivo early childhood caries medicine General Materials Science Microbiome Biology (General) QD1-999 Instrumentation Fluid Flow and Transfer Processes biology Physics Process Chemistry and Technology General Engineering Biofilm 030206 dentistry Engineering (General). Civil engineering (General) medicine.disease biology.organism_classification demineralization Computer Science Applications Demineralization Chemistry stomatognathic diseases biofilms TA1-2040 Fusobacterium nucleatum Early childhood caries |
| Zdroj: | Applied Sciences, Vol 11, Iss 3638, p 3638 (2021) Applied Sciences Volume 11 Issue 8 |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11083638 |
| Popis: | A previous longitudinal study about using microbiome as a caries indicator has successfully predicted early childhood caries (ECC) in healthy individuals, but there is no evidence to verify the composition of core microbiota and its pathogenicity in vitro and in vivo. Biofilm acidogenicity, S. mutans count, and biofilm composition were estimated by pH evaluation, colony-forming unit, and quantitative PCR, respectively. Extracellular polysaccharide production and enamel demineralization were observed by confocal laser scanning microscopy (CLSM) and transverse microradiography (TMR), respectively. A rat caries model was established for dental caries formation in vivo, and caries lesions were quantified by Keyes Scoring. We put forward that microbiota including Veillonella parvula, Fusobacterium nucleatum, Prevotella denticola, and Leptotrichia wadei served as the predictors for ECC may be the core microbiota in ECC. This study found that the core microbiota of ECC produced limited acid, but promoted growth and acidogenic ability of S. mutans. Besides, core microbiota could help to promote the development of biofilms. Moreover, the core microbiota enhanced the enamel demineralization in vitro and increased cariogenic potential in vivo. These results proved that core microbiota could promote the development of dental caries and plays an important role in the development of ECC. |
| Databáze: | OpenAIRE |
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