pH-sensitive chitosan nanoparticles loaded with dolutegravir as milk and food admixture for paediatric anti-HIV therapy

Autor: K. Priya Dharshini, Jin-Xuan Yang, B. N. Vedha Hari, Hao Fang, Rong-Hua Luo, D. Ramya Devi, Yong-Tang Zheng, Marek Brzeziński
Rok vydání: 2021
Předmět:
Drug
Magnetic Resonance Spectroscopy
Polymers and Plastics
Anti-HIV Agents
Pyridones
media_common.quotation_subject
Administration
Oral
HIV Infections
02 engineering and technology
010402 general chemistry
01 natural sciences
Piperazines
Cell Line
Chitosan
chemistry.chemical_compound
Biopolymers
Animal Shells
Oral administration
Crustacea
Oxazines
Materials Chemistry
Zeta potential
Animals
Humans
MTT assay
Particle Size
Child
Cytotoxicity
media_common
Drug Carriers
Organic Chemistry
Temperature
Spray Drying
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
0104 chemical sciences
Drug Liberation
Kinetics
Milk
chemistry
Food
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Drug delivery
Dolutegravir
Nanoparticles
0210 nano-technology
Heterocyclic Compounds
3-Ring
Nuclear chemistry
Zdroj: Carbohydrate Polymers. 256:117440
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2020.117440
Popis: The present study aims to develop Chitosan-based polymeric nanoparticles of anti-HIV drug Dolutegravir, to aid appropriate dose adjustment and ease of oral administration as milk and food admixture for children. The isolated Chitosan from the crab shell species Portunus Sanguinolentus has been characterized for their physicochemical properties. Nanoparticles were developed with varying ratio of drug: Chitosan and assessed for particle size (140-548 nm), zeta potential (+26.1 mV) with a maximum of 75 % drug content. Nanoparticles exhibited improved stability and drug release in the 0.1 N HCl medium compared to pure drug. The MTT assay and the Syncytia inhibition assay in C8166 (T-lymphatic cell line) infected with HIVIIIB viral strain, which showed better therapeutic efficiency and lesser cytotoxicity compared to the pure drug. In consonance with the data obtained, the use of chitosan from a novel source for drug delivery carrier has opened exceptional prospects for delivering drugs efficiently to paediatrics.
Databáze: OpenAIRE
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