Effects of microinjections of Group II metabotropic glutamate agents into the amygdala on sleep
| Autor: | Laurie L. Wellman, Enheng Dong, Larry D. Sanford, Linghui Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Agonist medicine.medical_specialty Microinjections medicine.drug_class Rapid eye movement sleep Sleep REM Receptors Metabotropic Glutamate Non-rapid eye movement sleep Article Internal medicine medicine Animals Amino Acids Rats Wistar Wakefulness Molecular Biology Dose-Response Relationship Drug Chemistry General Neuroscience Central nucleus of the amygdala Amygdala Bridged Bicyclo Compounds Heterocyclic Rats Stria terminalis Endocrinology Metabotropic receptor Xanthenes Neurology (clinical) Sleep onset Arousal Sleep Excitatory Amino Acid Antagonists Developmental Biology |
| Zdroj: | Brain Research. 1452:85-95 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2012.03.003 |
| Popis: | Systemic administration of the Group II metabotropic glutamate (mGlu) receptor agonist, LY379268 (LY37), dose-dependently suppresses rapid eye movement sleep (REM) whereas systemic administration of the mGlu II receptor antagonist, LY341495 (LY34), increases arousal. Group II mGlu receptors are highly expressed in the amygdala, a brain region involved in the regulation of sleep and arousal. To determine whether the amygdala is involved in mediating the effects of Group II mGlu agents on sleep, we microinjected LY37 and LY34 into the basal amygdala (BA) and the central nucleus of the amygdala (CNA) and recorded sleep and wakefulness. Wistar rats were implanted with electrodes for recording sleep and with bilateral cannulae aimed into BA for drug administration. Different groups of rats received bilateral microinjections of LY37 into BA at two dosage ranges (3.2 mM, 5.3 mM or 10.7 mM or 0.1 nM, 2.0 nM or 10.0 nM) or one dosage range of LY34 (1.0 nM, 30.0 nM or 60.0 nM). Microinjections into CNA were conducted at one dosage range for LY37 (0.1 nM, 2.0 nM or 10.0 nM) and for LY34 (1.0 nM, 30.0 nM or 60.0 nM). All drugs or vehicle alone were administered in a counterbalanced order at 5-day intervals. Following microinjection, sleep was recorded for 20 h. Microinjection of LY37 into BA at both nM and mM concentrations significantly decreased REM without significantly altering NREM, total sleep or wakefulness. The high dosage of LY34 in BA significantly suppressed NREM and total sleep. Microinjections of LY37 or LY34 into CNA had no significant impact on sleep. We suggest that Group II mGlu receptors may influence specific cells in BA that control descending output (via the CNA or bed nucleus of the stria terminalis) that in turn regulates pontine REM generator regions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect