Cationic Oligonucleotide−Peptide Conjugates with Aggregating Properties Enter Efficiently into Cells while Maintaining Hybridization Properties and Enzymatic Recognition
| Autor: | Gérard Nullans, Jean-Paul Behr, Guy Zuber, Bénédicte Pons, Andrew W. Fraley, Deniz Dalkara |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Protein Conformation Oligonucleotide Molecular Sequence Data Oligonucleotides Cationic polymerization Peptide General Chemistry Gene delivery Biochemistry Catalysis Colloid and Surface Chemistry Molecular recognition Protein structure chemistry Humans Nucleic Acid Conformation Amino Acid Sequence Peptides Peptide sequence HeLa Cells Conjugate |
| Zdroj: | Journal of the American Chemical Society. 128:10763-10771 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja060873e |
| Popis: | Oligonucleotide delivery is a crucial issue for therapeutical purposes and is often addressed by conjugation to short cationic peptides although with controversial results. To further examine this mechanism, a 15-mer anionic oligonucleotide was conjugated to a cationic peptide in order to obtain a diblock compound with an overall positive charge with aggregation properties. These microaggregates were efficiently internalized in cells via the expeditious pathway used by commercial gene delivery systems. Moreover, stability of the duplex formed with the complementary sequence increased without inhibiting oligonucleotide enzyme recognition as shown by the properties of the conjugate to prime chain elongation by Taq DNA polymerase in a linear amplification/sequencing process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|