Infection-enhancing lipopeptides do not improve intranasal immunization of cotton rats with a delta-G candidate live-attenuated human respiratory syncytial virus vaccine
| Autor: | Yvonne van Remmerden, Rik L. de Swart, D. Tien Nguyen, Albert D. M. E. Osterhaus, Jolande Boes, Teun Guichelaar, Selma Yüksel, Geert van Amerongen |
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| Přispěvatelé: | Virology |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_treatment
Immunology Short Report Respiratory Syncytial Virus Infections Antibodies Viral Vaccines Attenuated Virus Microbiology chemistry.chemical_compound Lipopeptides Adjuvants Immunologic Viral Envelope Proteins SDG 3 - Good Health and Well-being Nasopharynx medicine Respiratory Syncytial Virus Vaccines Immunology and Allergy Cationic Lipopeptides Animals Cotton rat Sigmodontinae Administration Intranasal Pharmacology biology Immunogenicity Lipopeptide Viral Load biology.organism_classification Virology Vaccination Disease Models Animal chemistry Respiratory Syncytial Virus Human Immunization Adjuvant |
| Zdroj: | Human Vaccines & Immunotherapeutics, 9(12), 2578-2583. Landes Bioscience |
| ISSN: | 2164-5515 |
| Popis: | Development of live-attenuated human respiratory syncytial virus (HRSV) vaccines has proven to be difficult. Several vaccine candidates were found to be over-attenuated and displayed limited immunogenicity. Recently, we identified three synthetic cationic lipopeptides that enhanced paramyxovirus infections in vitro. The infection enhancement proved to be mediated by enhanced virus binding to target cells. We hypothesized that these lipopeptides can be used as adjuvants to promote immune responses induced by live-attenuated paramyxovirus vaccines. This hypothesis was tested in a vaccination and challenge model in cotton rats, using a previously described recombinant live-attenuated candidate HRSV vaccine lacking the gene encoding the G glycoprotein (rHRSV Delta G). Surprisingly, intranasal vaccination of cotton rats with rHRSV.G formulated in infection-enhancing lipopeptides resulted in reduced virus loads in nasopharyngeal lavages, reduced seroconversion levels and reduced protection from wild-type HRSV challenge. In conclusion, we were unable to demonstrate the feasibility of lipopeptides as adjuvants for a candidate live-attenuated HRSV vaccine in the cotton rat model. |
| Databáze: | OpenAIRE |
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