Characterization of a new IgGλ myeloma plasma cell line (EJM): a further tool in the investigation of the biology of multiple myeloma
| Autor: | Ian M. Franklin, M. S. Hamilton, J. Ball, J. Lowe, E. Bromidge |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Myeloma protein medicine.medical_treatment Cytological Techniques Plasma Cells Alpha interferon Plasma cell Cell Line Immunoglobulin lambda-Chains medicine Ascitic Fluid Humans Multiple myeloma MHC class II biology Cell growth Hematology Middle Aged medicine.disease Molecular biology Phenotype Cytokine medicine.anatomical_structure Cell culture Immunoglobulin G Karyotyping biology.protein Female Multiple Myeloma |
| Zdroj: | British Journal of Haematology. 75:378-384 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1990.tb04352.x |
| Popis: | A new IgG lambda myeloma plasma cell line known as EJM was established from a peritoneal effusion from a patient with extramedullary myeloma. The EJM cells have a plasmablastic morphology with abundant rough endoplasmic reticulum and grow in liquid culture with a doubling time of 72 h and a labelling index of 36%. In addition to cytoplasmic IgG lambda, the cells are positive for CD9, 20, 32, 38, 44, 54, 71, 78, MHC Class II DR, DP and DQ. Studies on the control of the cell line proliferation by cytokines have demonstrated stimulation with interleukin 6. In contrast interferon alpha produces marked inhibition of proliferation in doses of greater than 100 units/ml. The culture conditions and the importance of accessory cells and cytokines in supporting myeloma plasma cell growth in vitro are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text