Estrella lausannensis, a new star in the Chlamydiales order

Autor: Guy Prod'hom, Gilbert Greub, Julia Lienard, Antony Croxatto
Rok vydání: 2011
Předmět:
DNA
Bacterial
Genes
rRNA/genetics
Sequence analysis
RNA
Ribosomal
16S/analysis
Immunology
Chlamydiae
Acanthamoeba
urologic and male genital diseases
medicine.disease_cause
Microbiology
23S ribosomal RNA
RNA
Ribosomal
16S
Chlamydiales/isolation & purification
medicine
Dictyostelium
Chlamydiaceae
DNA
Bacterial/analysis
Amoeba
Phylogeny
Chlamydia psittaci
Chlamydiales
RNA
Ribosomal
16S/genetics
biology
Amoeba/microbiology
Genes
rRNA
Sequence Analysis
DNA
Acanthamoeba/microbiology
Chlamydiales/genetics
biology.organism_classification
rpoB
Chlamydiales/classification
Coculture Techniques
DNA
Bacterial/genetics
Infectious Diseases
Microscopy
Fluorescence
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Dictyostelium/microbiology
Chlamydia trachomatis
Chlamydiales/growth & development
Zdroj: Microbes and Infection / Institut Pasteur, vol. 13, no. 14-15, pp. 1232-1241
ISSN: 1286-4579
DOI: 10.1016/j.micinf.2011.07.003
Popis: Originally, the Chlamydiales order was represented by a single family, the Chlamydiaceae, composed of several pathogens, such as Chlamydia trachomatis, Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia abortus. Recently, 6 new families of Chlamydia-related bacteria have been added to the Chlamydiales order. Most of these obligate intracellular bacteria are able to replicate in free-living amoebae. Amoebal co-culture may be used to selectively isolate amoeba-resisting bacteria. This method allowed in a previous work to discover strain CRIB 30, from an environmental water sample. Based on its 16S rRNA gene sequence similarity with Criblamydia sequanensis, strain CRIB 30 was considered as a new member of the Criblamydiaceae family. In the present work, phylogenetic analyses of the genes gyrA, gyrB, rpoA, rpoB, secY, topA and 23S rRNA as well as MALDI-TOF MS confirmed the taxonomic classification of strain CRIB 30. Morphological examination revealed peculiar star-shaped elementary bodies (EBs) similar to those of C. sequanensis. Therefore, this new strain was called "Estrella lausannensis". Finally, E. lausannensis showed a large amoebal host range and a very efficient replication rate in Acanthamoeba species. Furthermore, E. lausannensis is the first member of the Chlamydiales order to grow successfully in the genetically tractable Dictyostelium discoideum, which opens new perspectives in the study of chlamydial biology.
Databáze: OpenAIRE
Externí odkaz: