Neutrophil-Mediated Proteolysis of Thrombospondin-1 Promotes Platelet Adhesion and String Formation

Autor: Nahla Ibrahim, Lisa-Marie Mauracher, Lejla Alidzanovic, Lena Hell, Bernd Jilma, Alice Assinger, Ulrich Budde, Johannes A. Schmid, Manuel Salzmann, Ingrid Pabinger, Barbara Tischler, Sabine Rauscher, Christine Brostjan, Patrick Starlinger, Katharina Seif, Branislav Zagrapan
Rok vydání: 2018
Předmět:
Blood Platelets
0301 basic medicine
proteolysis
endocrine system
Proteases
Platelet Aggregation
Neutrophils
Proteolysis
cathepsin G
030204 cardiovascular system & hematology
Cathepsin G
Thrombospondin 1
Mice
03 medical and health sciences
chemistry.chemical_compound
Platelet Adhesiveness
0302 clinical medicine
Von Willebrand factor
immune system diseases
von Willebrand Factor
Cellular Haemostasis and Platelets
medicine
Animals
Humans
Platelet
thrombospondin-1
Cells
Cultured

Mice
Knockout

Hemostasis
biology
medicine.diagnostic_test
Elastase
platelet string formation
virus diseases
Hematology
Neutrophil extracellular traps
Coculture Techniques
Cell biology
Mice
Inbred C57BL

030104 developmental biology
chemistry
biology.protein
platelet adhesion
Endothelium
Vascular

Protein Multimerization
neutrophil elastase
Zdroj: Thrombosis and Haemostasis
ISSN: 2567-689X
0340-6245
DOI: 10.1055/s-0038-1675229
Popis: Thrombospondin-1 (TSP-1) is primarily expressed by platelets and endothelial cells (ECs) and rapidly released upon their activation. It functions in haemostasis as a bridging molecule in platelet aggregation, by promoting platelet adhesion to collagen and by protecting von Willebrand factor strings from degradation. In blood of patients undergoing surgery and in co-cultures of neutrophils with platelets or ECs, we observed proteolysis of the 185 kDa full-length TSP-1 to a 160-kDa isoform. We hypothesized that TSP-1 processing may alter its haemostatic properties. Selective enzyme inhibitors in co-cultures revealed that neutrophil proteases elastase and cathepsin G mediate TSP-1 processing. The cut site of cathepsin G was mapped to TSP-1 amino acids R237/T238 by Edman sequencing. Formation of neutrophil extracellular traps protected TSP-1 from complete degradation and promoted controlled processing to the 160-kDa isoform. Haemostatic properties were tested by platelet aggregation, adhesion, coagulation and string formation under flow. Platelets from TSP-1 deficient mice did not differ from wild-type in platelet aggregation but showed severe impairment of platelet adhesion to collagen and string formation under flow. Reconstitution experiments revealed that the 160-kDa TSP-1 isoform was markedly more potent than the 185-kDa full-length molecule in restoring function. Thus, TSP-1 processing by neutrophil proteases yields a 160-kDa isoform which shows enhanced potency to promote platelet adhesion and string formation. This finding reveals a novel mechanism of neutrophil-mediated thrombus formation and provides first evidence for the impact of TSP-1 proteolysis on its haemostatic properties.
Databáze: OpenAIRE