Prevalence of exon 11 internal tandem duplications in the C-KIT proto-oncogene in Australian canine mast cell tumours
| Autor: | RJ Mccoy, A. E. Peaston, Elizabeth C. Dobson, AE Kessell, L Brown, TS Smith, D Mitrovic, Vanessa S. Tamlin, Michelle L. Hebart |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Skin Neoplasms 040301 veterinary sciences Patnaik grade III Biology law.invention 0403 veterinary science 03 medical and health sciences Exon Dogs law medicine Prevalence Animals Dog Diseases Mast Cells Grading (tumors) Polymerase chain reaction General Veterinary Oncogene Australia Mastocytoma Histology 04 agricultural and veterinary sciences General Medicine Exons medicine.disease Mast cell Proto-Oncogene Proteins c-kit 030104 developmental biology medicine.anatomical_structure |
| Zdroj: | Australian veterinary journal. 95(10) |
| ISSN: | 1751-0813 |
| Popis: | Objective To measure the prevalence of internal tandem duplications (ITDs) in exon 11 of the proto-oncogene C-KIT in a sample of Australian cutaneous canine mast cell tumours (MCTs) drawn from general practice and to evaluate relationships between tumour mutation status and prognostic factors including signalment, tumour histological grade, tumour anatomical location and tumour size. Methods C-KIT exon 11 ITDs were detected by PCR in DNA extracted from formalin-fixed, paraffin-embedded canine MCTs sourced from three veterinary diagnostic laboratories in Adelaide and Melbourne. Tumours were graded according to two different systems (Patnaik and Kiupel systems) by board-certified anatomical pathologists blinded to the PCR results. Relationships between tumour mutation status and prognostic factors were evaluated using a generalised binary logistic regression analysis. Results ITDs were identified in 13 of 74 cutaneous canine MCT samples, giving an overall prevalence of 17.6% (95% confidence interval: 8.9–26.2%). ITDs were detected in 10 of 18 Patnaik grade III MCTs (55.6%) and 11 of 22 Kiupel high-grade MCTs (50%). Wald chi-square analysis revealed that detection of tumour ITDs was significantly associated with both Patnaik’s and Kiupel’s histologic grading systems (each: P |
| Databáze: | OpenAIRE |
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