Endoscopic appearance of GERD: putative role of cell proliferation
| Autor: | Carlo Calabrese, Giovanna Cenacchi, G. Di Febo, A. Fabbri, Davide Treré, Massimo Derenzini, Manuela Vici |
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| Přispěvatelé: | C. Calabrese, D. Trere, A. Fabbri, G. Cenacchi, M. Vici, M. Derenzini, G. Di Febo |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Nerd Biopsy Ubiquitin-Protein Ligases Video Recording Regurgitation (circulation) Gastroenterology Severity of Illness Index Endoscopy Gastrointestinal Gastric Acid ENDOSCOPIC APPEARANCE Esophagus Microscopy Electron Transmission Internal medicine medicine Humans Single-Blind Method Intestinal Mucosa Aged Cell Proliferation Retrospective Studies Aged 80 and over MIB1 Hepatology medicine.diagnostic_test business.industry digestive oral and skin physiology Reflux Heartburn Histology GERD Gastric Acidity Determination Middle Aged medicine.disease Prognosis digestive system diseases Endoscopy Disease Progression Gastroesophageal Reflux Female medicine.symptom business Immunostaining Follow-Up Studies |
| Zdroj: | Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver. 39(8) |
| ISSN: | 1590-8658 |
| Popis: | Background Erosive esophagitis is a frequent endoscopic feature in patients with gastro-oesophageal reflux disease. However, most of patients with heartburn/regurgitation have a non-erosive reflux disease. The reason for this heterogeneous impact of gastro-oesophageal reflux disease on oesophageal mucosa is unknown to date. Aim To evaluate the cell proliferation status of oesophageal epithelium in both healthy normal subjects and patients with gastro-oesophageal reflux disease with or without erosions. Materials and methods All the subjects underwent endoscopy and biopsies were taken at 5 cm from the squamo-columnar junction. Specimens were analysed both at histology and at transmission electron microscopy. Cell proliferation was evaluated by MIB1 immunostaining. Of the 85 subjects were studied, 10 were healthy controls with normal pH-testing and macroscopical, histological and ultrastructural patterns; 37were patients with erosive esophagitis, and 38 patients with non-erosive reflux disease. Results At histology, of the 37 patients affected by erosive esophagitis, 30 had normal mucosa and 7 showed mild oesophagitis. One patient with non-erosive reflux disease showed signs of oesophagitis at histology. At TEM, all patients with gastro-oesophageal reflux disease had ultrastructural patterns of damage i.e. dilations of intercellular spaces (DIS), and all controls had a normal ultrastructural pattern. The mean (±SD) MIB1-LI values of normal subjects and non-erosive reflux disease and erosive oesophagitis patients were 62.2% (±9.1), 29.7% (±7.2) and 16.2% (±5.2), respectively; there were significant differences among the three groups (p Conclusions Oesophageal mucosa of patients with reflux symptoms presents a decrease in MIB1 immunostaining of 50% and 25% in non-erosive reflux disease and erosive esophagitis patients with respect to normal subjects. |
| Databáze: | OpenAIRE |
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