Microbial and host immune factors as drivers of COPD
| Autor: | Izabela Nita, Seraina Martina Beyeler, Sebastian Robert Ott, Laura Ursina Morf, Benjamin J. Marsland, Markus Hilty, Eric Bernasconi, Christophe von Garnier, Weihong Qi, Moana Mika |
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| Přispěvatelé: | University of Zurich |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine 610 Medicine & health 10071 Functional Genomics Center Zurich 03 medical and health sciences 0302 clinical medicine Gene expression medicine COPD Macrophage medicine.diagnostic_test business.industry Host (biology) medicine.disease Obstructive lung disease respiratory tract diseases 3. Good health 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure 030228 respiratory system Immunology 570 Life sciences biology Original Article Analysis of variance business Respiratory tract |
| Zdroj: | ERJ Open Research Mika, Moana; Nita, Izabela Magdalena; Morf, Laura Ursina; Qi, Weihong; Beyeler, Seraina Martina; Bernasconi, Eric; Marsland, Benjamin J; Ott, Sebastian Robert; von Garnier, Christophe; Hilty, Markus (2018). Microbial and host immune factors as drivers of COPD. ERJ Open Research, 4(3) European Respiratory Society 10.1183/23120541.00015-2018 ERJ open research, vol. 4, no. 3, pp. 1-10 ERS Monograph, 4 (3) |
| ISSN: | 2312-0541 |
| Popis: | Compartmentalisation of the respiratory tract microbiota in patients with different chronic obstructive pulmonary disease (COPD) severity degrees needs to be systematically investigated. In addition, it is unknown if the inflammatory and emphysematous milieux in patients with COPD are associated with changes in the respiratory tract microbiota and host macrophage gene expression. We performed a cross-sectional study to compare non-COPD controls (n=10) to COPD patients (n=32) with different disease severity degrees. Samples (n=187) were obtained from different sites of the upper and lower respiratory tract. Microbiota analyses were performed by 16S ribosomal RNA gene sequencing and host gene expression analyses by quantitative real-time PCR of distinct markers of bronchoalveolar lavage cells. Overall, the microbial communities of severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) grade 3/4) patients clustered significantly differently to controls and less severe COPD (GOLD 1/2) patients (permutational multivariate ANOVA (MANOVA), p=0.001). However, we could not detect significant associations between the different sampling sites in the lower airways. In addition, the chosen set of host gene expression markers significantly separated COPD GOLD 3/4 patients, and we found correlations between the composition of the microbiota and the host data. In conclusion, this study demonstrates associations between host gene expression and microbiota profiles that may influence the course of COPD. Associations of the host immune response and a disordered microbiota in patients with different COPD severity degrees http://ow.ly/h2mW30k9Nua |
| Databáze: | OpenAIRE |
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