Systemic administration of the chemokine macrophage inflammatory protein 1 exacerbates inflammatory bowel disease in a mouse model
| Autor: | M. Buckley, R. Pettipher, V. Chance, Thomas T. MacDonald, Sylvia L.F. Pender, M. Edwards, C.V. Whiting |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Colon medicine.medical_treatment Inflammatory bowel disease Interferon-gamma Mice medicine Animals Intestinal Mucosa Colitis Chemokine CCL4 Macrophage inflammatory protein Cells Cultured Chemokine CCL3 Mice Inbred BALB C Lamina propria biology Tumor Necrosis Factor-alpha business.industry Inflammatory Bowel Disease Gastroenterology Fibroblasts Macrophage Inflammatory Proteins medicine.disease Immunohistochemistry Ulcerative colitis Disease Models Animal medicine.anatomical_structure Cytokine Immunology biology.protein Colitis Ulcerative Female Matrix Metalloproteinase 3 Tumor necrosis factor alpha business Injections Intraperitoneal |
| Zdroj: | Gut. 54:1114-1120 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.2004.052779 |
| Popis: | Exacerbations of inflammatory bowel disease are thought to be related to concurrent infections. As infections are associated with elevated local and serum concentrations of chemokines, we have determined whether systemic administration of the CC chemokine macrophage inflammatory protein 1alpha (MIP-1alpha) exacerbates colitis in a mouse model.Colitis was induced in Balb/c mice using trinitrobenzene sulfonic acid (TNBS). Starting four days later, animals received daily intraperitoneal injections of recombinant MIP-1alpha. On day 7, mice were killed and pieces of colon taken for immunohistology and polymerase chain reaction analysis. The direct effects of MIP-1alpha on mucosal T cells and fibroblasts in vitro were also investigated.Systemic administration of MIP-1alpha markedly enhanced colitis with mice developing large transmural ulcers filled with granulation tissue. Treatment resulted in increased numbers of CD4 cells infiltrating the colonic lamina propria, increased interferon gamma (IFN-gamma) levels, and increased transcripts for tumour necrosis factor alpha (TNF-alpha) and matrix metalloproteinase 3 (MMP3). Isolated lamina propria lymphocytes from mice with TNBS colitis contained increased numbers of IFN-gamma and TNF-alpha transcripts when stimulated with MIP-1alpha in vitro. Colonic lamina propria fibroblasts also responded to MIP-1alpha with increased proliferation and decreased collagen 1 synthesis but fibroblast proliferation was not seen in vivo.These experiments show that increasing serum concentrations of a chemokine, MIP-1alpha, exacerbates immune mediated colitis. The effect seems to be due to the ability of MIP-1alpha to boost Th1 responses in the gut wall. Our findings also suggest a potential pathway by which peripheral infections can exacerbate inflammatory bowel disease. |
| Databáze: | OpenAIRE |
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