| Popis: |
Background:The search for a better therapeutic approach in wound healing continues to be made by current researchers. Adipose-Derived Stem Cells Conditioned Medium (ASCs-CM), through its paracrine mechanism, showed a promising result in enhancing wound healing. This study investigated the effect of locally injected ASCs-CM on re-epithelialization, fibroblasts count, capillary density, and collagen thickness, compared to conventional framycetin gauze dressing in Wistar rats treated with full-thickness acute wound model. Methods:40 adult Wistar rats weighing between 150 and 250 grams were used in this study to establish a full-thickness wound model. They were randomly divided into 2 groups: locally injected ASCs-CM group and framycetin gauze dressing group. The outcomes were measured on days 3, 7, 14, and 21. Data were analyzed with a T-test and Mann-Whitney as an alternative. Results:Local injection of ASCs-CM increased re-epithelialization, fibroblasts count, capillary density, and collagen thickness by 10.84, 44.90, 4.75, and 21.79, respectively. The locally injected wound group showed a higher number of all outcomes in every observation day, except for re-epithelialization on day 21, by which time the wounds in both groups were already healed. Conclusion:Administration of locally injected ASCs-CM to a full-thickness open acute wound model showed satisfactory results. This can be seen from the faster clinical re-epithelialization process - the more fibroblasts formed, the more angiogenesis formed and yielded thicker collagen synthesis compared to those treated with framycetin gauze dressings. |
