Membrane formation by interfacial cross-linking of chitosan for microencapsulation ofLactococcus lactis
| Autor: | G. D. Darling, Denis Poncelet, Ronald J. Neufeld, Claude P. Champagne, A. Groboillot |
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| Rok vydání: | 1993 |
| Předmět: |
biology
Lactococcus lactis technology industry and agriculture Bioengineering macromolecular substances biology.organism_classification Applied Microbiology and Biotechnology Interfacial polymerization Lactic acid carbohydrates (lipids) Chitosan chemistry.chemical_compound Membrane chemistry Biochemistry Chemical engineering Hexamethylene diisocyanate Fermentation Glutaraldehyde Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 42:1157-1163 |
| ISSN: | 1097-0290 0006-3592 |
| Popis: | Lactic acid bacteria were microencapsulated within cross-linked chitosan membranes formed by emulsification/interfacial polymerization. The technique was modified and optimized to provide biocompatible conditions during encapsulation involving the use of mineral oils as the continuous phase and chitosan as the membrane material. Chitosan cross-linked with hexamethylene diisocyanate or glutaraldehyde resulted in strong membranes, with a narrow size distribution about a mean diameter of 150 mum. Cell viability and activity was demonstrated by the acidification of milk. Loss of acidification activity during microencapsulation was recovered in subsequent fermentations to levels similar to that of free cell fermentations. |
| Databáze: | OpenAIRE |
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