Dzip3 regulates developmental genes in mouse embryonic stem cells by reorganizing 3D chromatin conformation
| Autor: | Yuko Imamura, Masamichi Doiguchi, Miki Higashi, Satoshi Fujii, Takeya Nakagawa, Daishi Inoue, Tatsuharu Sato, Akihiko Okuda, Takashi Ito, Mitsuhiro Yoneda, Hirofumi Mizusaki, Takayuki Miyanishi, Hitoshi Aihara |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
animal structures
Ubiquitin-Protein Ligases Cellular differentiation Gene Expression Article Epigenesis Genetic Histones Mice Ubiquitin Gene expression Histone H2A Transcriptional regulation Animals Genes Developmental Cells Cultured Binding Sites Multidisciplinary biology Ubiquitination RNA-Binding Proteins Cell Differentiation Mouse Embryonic Stem Cells Chromatin Assembly and Disassembly Embryonic stem cell Chromatin Cell biology Ubiquitin ligase biology.protein Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep16567 |
| Popis: | In mouse embryonic stem (mES) cells, ubiquitylation of histone H2A lysine 119 represses a large number of developmental genes and maintains mES cell pluripotency. It has been suggested that a number of H2A ubiquitin ligases as well as deubiquitylases and related peptide fragments contribute to a delicate balance between self-renewal and multi-lineage differentiation in mES cells. Here, we tested whether known H2A ubiquitin ligases and deubiquitylases are involved in mES cell regulation and discovered that Dzip3, the E3 ligase of H2AK119, represses differentiation-inducible genes, as does Ring1B. The two sets of target genes partially overlapped but had different spectra. We found that Dzip3 represses gene expression by orchestrating changes in 3D organization, in addition to regulating ubiquitylation of H2A. Our results shed light on the epigenetic mechanism of transcriptional regulation, which depends on 3D chromatin reorganization to regulate mES cell differentiation. Scientific Reports, 5, 16567; 2015 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|